Akkermansia muciniphila ameliorates the age-related decline in colonic mucus thickness and attenuates immune activation in accelerated aging Ercc1(-/7) mice

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van der Lugt , B , van Beek , A A , Aalvink , S , Meijer , B , Sovran , B , Vermeij , W P , Brandt , R M C , de Vos , W M , Savelkoul , H F J , Steegenga , W T & Belzer , C 2019 , ' Akkermansia muciniphila ameliorates the age-related decline in colonic mucus thickness and attenuates immune activation in accelerated aging Ercc1(-/7) mice ' , Immunity & Ageing , vol. 16 , 6 . https://doi.org/10.1186/s12979-019-0145-z

Title: Akkermansia muciniphila ameliorates the age-related decline in colonic mucus thickness and attenuates immune activation in accelerated aging Ercc1(-/7) mice
Author: van der Lugt, Benthe; van Beek, Adriaan A.; Aalvink, Steven; Meijer, Ben; Sovran, Bruno; Vermeij, Wilbert P.; Brandt, Renata M. C.; de Vos, Willem M.; Savelkoul, Huub F. J.; Steegenga, Wilma T.; Belzer, Clara
Other contributor: University of Helsinki, Department of Bacteriology and Immunology





Date: 2019-03-08
Language: eng
Number of pages: 17
Belongs to series: Immunity & Ageing
ISSN: 1742-4933
DOI: https://doi.org/10.1186/s12979-019-0145-z
URI: http://hdl.handle.net/10138/300299
Abstract: BackgroundThe use of Akkermansia muciniphila as potential therapeutic intervention is receiving increasing attention. Health benefits attributed to this bacterium include an improvement of metabolic disorders and exerting anti-inflammatory effects. The abundance of A. muciniphila is associated with a healthy gut in early mid- and later life. However, the effects of A. muciniphila on a decline in intestinal health during the aging process are not investigated yet. We supplemented accelerated aging Ercc1(-/7) mice with A. muciniphila for 10weeks and investigated histological, transcriptional and immunological aspects of intestinal health.ResultsThe thickness of the colonic mucus layer increased about 3-fold after long-term A. muciniphila supplementation and was even significantly thicker compared to mice supplemented with Lactobacillus plantarum WCFS1. Colonic gene expression profiles pointed towards a decreased expression of genes and pathways related to inflammation and immune function, and suggested a decreased presence of B cells in colon. Total B cell frequencies in spleen and mesenteric lymph nodes were not altered after A. muciniphila supplementation. Mature and immature B cell frequencies in bone marrow were increased, whereas B cell precursors were unaffected. These findings implicate that B cell migration rather than production was affected by A. muciniphila supplementation. Gene expression profiles in ileum pointed toward a decrease in metabolic- and immune-related processes and antimicrobial peptide production after A. muciniphila supplementation. Besides, A. muciniphila decreased the frequency of activated CD80(+)CD273(-) B cells in Peyer's patches. Additionally, the increased numbers of peritoneal resident macrophages and a decrease in Ly6C(int) monocyte frequencies in spleen and mesenteric lymph nodes add evidence for the potentially anti-inflammatory properties of A. muciniphila.ConclusionsAltogether, we show that supplementation with A. muciniphila prevented the age-related decline in thickness of the colonic mucus layer and attenuated inflammation and immune-related processes at old age. This study implies that A. muciniphila supplementation can contribute to a promotion of healthy aging.
Subject: Aging
Akkermansia muciniphila
Intestinal immunity
Mucus layer
Intestinal barrier
GUT MICROBIOTA
GENE-EXPRESSION
HOST
BACTERIAL
INFLAMMATION
ENTEROCYTES
METABOLITES
PHYSIOLOGY
INTEGRITY
COLITIS
3121 General medicine, internal medicine and other clinical medicine
3111 Biomedicine
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