Akkermansia muciniphila ameliorates the age-related decline in colonic mucus thickness and attenuates immune activation in accelerated aging Ercc1(-/7) mice

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dc.contributor.author van der Lugt, Benthe
dc.contributor.author van Beek, Adriaan A.
dc.contributor.author Aalvink, Steven
dc.contributor.author Meijer, Ben
dc.contributor.author Sovran, Bruno
dc.contributor.author Vermeij, Wilbert P.
dc.contributor.author Brandt, Renata M. C.
dc.contributor.author de Vos, Willem M.
dc.contributor.author Savelkoul, Huub F. J.
dc.contributor.author Steegenga, Wilma T.
dc.contributor.author Belzer, Clara
dc.date.accessioned 2019-03-22T08:10:01Z
dc.date.available 2019-03-22T08:10:01Z
dc.date.issued 2019-03-08
dc.identifier.citation van der Lugt , B , van Beek , A A , Aalvink , S , Meijer , B , Sovran , B , Vermeij , W P , Brandt , R M C , de Vos , W M , Savelkoul , H F J , Steegenga , W T & Belzer , C 2019 , ' Akkermansia muciniphila ameliorates the age-related decline in colonic mucus thickness and attenuates immune activation in accelerated aging Ercc1(-/7) mice ' , Immunity & Ageing , vol. 16 , 6 . https://doi.org/10.1186/s12979-019-0145-z
dc.identifier.other PURE: 123454143
dc.identifier.other PURE UUID: 574a9be0-a620-4f7d-8200-4204870b636a
dc.identifier.other WOS: 000460842000001
dc.identifier.other Scopus: 85062728314
dc.identifier.uri http://hdl.handle.net/10138/300299
dc.description.abstract BackgroundThe use of Akkermansia muciniphila as potential therapeutic intervention is receiving increasing attention. Health benefits attributed to this bacterium include an improvement of metabolic disorders and exerting anti-inflammatory effects. The abundance of A. muciniphila is associated with a healthy gut in early mid- and later life. However, the effects of A. muciniphila on a decline in intestinal health during the aging process are not investigated yet. We supplemented accelerated aging Ercc1(-/7) mice with A. muciniphila for 10weeks and investigated histological, transcriptional and immunological aspects of intestinal health.ResultsThe thickness of the colonic mucus layer increased about 3-fold after long-term A. muciniphila supplementation and was even significantly thicker compared to mice supplemented with Lactobacillus plantarum WCFS1. Colonic gene expression profiles pointed towards a decreased expression of genes and pathways related to inflammation and immune function, and suggested a decreased presence of B cells in colon. Total B cell frequencies in spleen and mesenteric lymph nodes were not altered after A. muciniphila supplementation. Mature and immature B cell frequencies in bone marrow were increased, whereas B cell precursors were unaffected. These findings implicate that B cell migration rather than production was affected by A. muciniphila supplementation. Gene expression profiles in ileum pointed toward a decrease in metabolic- and immune-related processes and antimicrobial peptide production after A. muciniphila supplementation. Besides, A. muciniphila decreased the frequency of activated CD80(+)CD273(-) B cells in Peyer's patches. Additionally, the increased numbers of peritoneal resident macrophages and a decrease in Ly6C(int) monocyte frequencies in spleen and mesenteric lymph nodes add evidence for the potentially anti-inflammatory properties of A. muciniphila.ConclusionsAltogether, we show that supplementation with A. muciniphila prevented the age-related decline in thickness of the colonic mucus layer and attenuated inflammation and immune-related processes at old age. This study implies that A. muciniphila supplementation can contribute to a promotion of healthy aging. en
dc.format.extent 17
dc.language.iso eng
dc.relation.ispartof Immunity & Ageing
dc.rights unspecified
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Aging
dc.subject Akkermansia muciniphila
dc.subject Intestinal immunity
dc.subject Mucus layer
dc.subject Intestinal barrier
dc.subject HOST
dc.subject BACTERIAL
dc.subject ENTEROCYTES
dc.subject METABOLITES
dc.subject PHYSIOLOGY
dc.subject INTEGRITY
dc.subject COLITIS
dc.subject 3121 General medicine, internal medicine and other clinical medicine
dc.subject 3111 Biomedicine
dc.title Akkermansia muciniphila ameliorates the age-related decline in colonic mucus thickness and attenuates immune activation in accelerated aging Ercc1(-/7) mice en
dc.type Article
dc.contributor.organization Department of Bacteriology and Immunology
dc.contributor.organization Willem Meindert Vos de / Principal Investigator
dc.contributor.organization Medicum
dc.contributor.organization Research Programs Unit
dc.contributor.organization Immunobiology Research Program
dc.contributor.organization Helsinki University Hospital Area
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1186/s12979-019-0145-z
dc.relation.issn 1742-4933
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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