Comparison of serum serotonin and serum 5-HIAA LC-MS/MS assays in the diagnosis of serotonin producing neuroendocrine neoplasms : A pilot study

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Lindström , M , Tohmola , N , Renkonen , R , Hämäläinen , E , Schalin-Jäntti , C & Itkonen , O 2018 , ' Comparison of serum serotonin and serum 5-HIAA LC-MS/MS assays in the diagnosis of serotonin producing neuroendocrine neoplasms : A pilot study ' , Clinica Chimica Acta , vol. 482 , pp. 78-83 . https://doi.org/10.1016/j.cca.2018.03.030

Title: Comparison of serum serotonin and serum 5-HIAA LC-MS/MS assays in the diagnosis of serotonin producing neuroendocrine neoplasms : A pilot study
Author: Lindström, Mikael; Tohmola, Niina; Renkonen, Risto; Hämäläinen, Esa; Schalin-Jäntti, Camilla; Itkonen, Outi
Contributor: University of Helsinki, HUSLAB
University of Helsinki, HUSLAB
University of Helsinki, HUSLAB
University of Helsinki, Clinicum
University of Helsinki, HUSLAB
Date: 2018-07
Language: eng
Number of pages: 6
Belongs to series: Clinica Chimica Acta
ISSN: 0009-8981
URI: http://hdl.handle.net/10138/300392
Abstract: Background: Serotonin (5-hydroxytyramine) is a mediator of gastrointestinal smooth muscle contraction, and is secreted by neuroendocrine neoplasms (NENs). We developed a liquid chromatography tandem mass spectrometry (LC-MS/MS) assay for serum serotonin to be used in NEN diagnostics and follow-up. Methods: We used serum samples from healthy volunteers (n = 31) and patients suspected or monitored for NEN (n = 98). Serotonin-D-4 internal standard was added to samples before solid phase extraction (SPE) and quantification by LC-MS/MS. The effects of sample handling and preparation on serotonin stability were studied. Finally, we established a provisional reference range for serum serotonin and compared our assay with serum 5hydroxyindoleacetic acid (5-HIAA) for detection of NENs. Results: Our assay is sensitive and has a wide linear range (10-10,000 nmo1/1). Serum serotonin is stable for 7 days at room temperature and for 3 months at -20 degrees C. Sampling temperature is not critical. Normal range for serum serotonin was 270-1490 nmo1/1. We found that serum serotonin and 5-HIAA performed equally well as diagnostic tests for NENs. Conclusions: Our LC-MS/MS assay for serum serotonin is well suited for clinical research and patient diagnostics. Our results confirm that it can complement 5-HIAA in diagnosis of NENs.
Subject: Serotonin
5-HIAA
NEN
Neuroendocrine neoplasm
LC-MS/MS
TANDEM MASS-SPECTROMETRY
POOR PLASMA SEROTONIN
PLATELET-RICH PLASMA
LIQUID-CHROMATOGRAPHY
CARCINOID-TUMORS
URINE
METABOLITES
ACID
3111 Biomedicine
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