Sodupe Ortega , E , Sanz-Garcia , A , Pernia-Espinoza , A & Escobedo-Lucea , C 2019 , ' Efficient Fabrication of Polycaprolactone Scaffolds for Printing Hybrid Tissue-Engineered Constructs ' , Materials , vol. 12 , no. 4 , 613 . https://doi.org/10.3390/ma12040613
Title: | Efficient Fabrication of Polycaprolactone Scaffolds for Printing Hybrid Tissue-Engineered Constructs |
Author: | Sodupe Ortega, Enrique; Sanz-Garcia, Andres; Pernia-Espinoza, Alpha; Escobedo-Lucea, Carmen |
Contributor organization: | Divisions of Faculty of Pharmacy Division of Pharmaceutical Biosciences Drug Research Program |
Date: | 2019-02-02 |
Language: | eng |
Number of pages: | 18 |
Belongs to series: | Materials |
ISSN: | 1996-1944 |
DOI: | https://doi.org/10.3390/ma12040613 |
URI: | http://hdl.handle.net/10138/300498 |
Abstract: | Hybrid constructs represent substantial progress in tissue engineering (TE) towards producing implants of a clinically relevant size that recapitulate the structure and multicellular complexity of the native tissue. They are created by interlacing printed scaffolds, sacrificial materials, and cell-laden hydrogels. A suitable biomaterial is a polycaprolactone (PCL); however, due to the higher viscosity of this biopolymer, three-dimensional (3D) printing of PCL is slow, so reducing PCL print times remains a challenge. We investigated parameters, such as nozzle shape and size, carriage speed, and print temperature, to find a tradeoff that speeds up the creation of hybrid constructs of controlled porosity. We performed experiments with conical, cylindrical, and cylindrical shortened nozzles and numerical simulations to infer a more comprehensive understanding of PCL flow rate. We found that conical nozzles are advised as they exhibited the highest shear rate, which increased the flow rate. When working at a low carriage speed, conical nozzles of a small diameter tended to form-flatten filaments and became highly inefficient. However, raising the carriage speed revealed shortcomings because passing specific values created filaments with a heterogeneous diameter. Small nozzles produced scaffolds with thin strands but at long building times. Using large nozzles and a high carriage speed is recommended. Overall, we demonstrated that hybrid constructs with a clinically relevant size could be much more feasible to print when reaching a tradeoff between temperature, nozzle diameter, and speed. |
Subject: |
tissue engineering
polycaprolactone porous scaffolds hybrid constructs 3D printing MOLECULAR-WEIGHT DEGRADATION 116 Chemical sciences 216 Materials engineering 317 Pharmacy |
Peer reviewed: | Yes |
Rights: | cc_by |
Usage restriction: | openAccess |
Self-archived version: | publishedVersion |
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