Efficient Fabrication of Polycaprolactone Scaffolds for Printing Hybrid Tissue-Engineered Constructs

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http://hdl.handle.net/10138/300498

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Sodupe Ortega , E , Sanz-Garcia , A , Pernia-Espinoza , A & Escobedo-Lucea , C 2019 , ' Efficient Fabrication of Polycaprolactone Scaffolds for Printing Hybrid Tissue-Engineered Constructs ' , Materials , vol. 12 , no. 4 , 613 . https://doi.org/10.3390/ma12040613

Title: Efficient Fabrication of Polycaprolactone Scaffolds for Printing Hybrid Tissue-Engineered Constructs
Author: Sodupe Ortega, Enrique; Sanz-Garcia, Andres; Pernia-Espinoza, Alpha; Escobedo-Lucea, Carmen
Contributor: University of Helsinki, Divisions of Faculty of Pharmacy
University of Helsinki, Division of Pharmaceutical Biosciences
Date: 2019-02-02
Language: eng
Number of pages: 18
Belongs to series: Materials
ISSN: 1996-1944
URI: http://hdl.handle.net/10138/300498
Abstract: Hybrid constructs represent substantial progress in tissue engineering (TE) towards producing implants of a clinically relevant size that recapitulate the structure and multicellular complexity of the native tissue. They are created by interlacing printed scaffolds, sacrificial materials, and cell-laden hydrogels. A suitable biomaterial is a polycaprolactone (PCL); however, due to the higher viscosity of this biopolymer, three-dimensional (3D) printing of PCL is slow, so reducing PCL print times remains a challenge. We investigated parameters, such as nozzle shape and size, carriage speed, and print temperature, to find a tradeoff that speeds up the creation of hybrid constructs of controlled porosity. We performed experiments with conical, cylindrical, and cylindrical shortened nozzles and numerical simulations to infer a more comprehensive understanding of PCL flow rate. We found that conical nozzles are advised as they exhibited the highest shear rate, which increased the flow rate. When working at a low carriage speed, conical nozzles of a small diameter tended to form-flatten filaments and became highly inefficient. However, raising the carriage speed revealed shortcomings because passing specific values created filaments with a heterogeneous diameter. Small nozzles produced scaffolds with thin strands but at long building times. Using large nozzles and a high carriage speed is recommended. Overall, we demonstrated that hybrid constructs with a clinically relevant size could be much more feasible to print when reaching a tradeoff between temperature, nozzle diameter, and speed.
Subject: tissue engineering
polycaprolactone
porous scaffolds
hybrid constructs
3D printing
MOLECULAR-WEIGHT
DEGRADATION
116 Chemical sciences
216 Materials engineering
317 Pharmacy
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