Light-Triggered Cellular Delivery of Oligonucleotides

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http://hdl.handle.net/10138/300499

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Kontturi , L-S , van den Dikkenberg , J , Urtti , A , Hennink , W E & Mastrobattista , E 2019 , ' Light-Triggered Cellular Delivery of Oligonucleotides ' , Pharmaceutics , vol. 11 , no. 2 , 90 . https://doi.org/10.3390/pharmaceutics11020090

Title: Light-Triggered Cellular Delivery of Oligonucleotides
Author: Kontturi, Leena-Stiina; van den Dikkenberg, Joep; Urtti, Arto; Hennink, Wim E.; Mastrobattista, Enrico
Contributor: University of Helsinki, Divisions of Faculty of Pharmacy
University of Helsinki, Drug Research Program
Date: 2019-02
Language: eng
Number of pages: 16
Belongs to series: Pharmaceutics
ISSN: 1999-4923
URI: http://hdl.handle.net/10138/300499
Abstract: The major challenge in the therapeutic applicability of oligonucleotide-based drugs is the development of efficient and safe delivery systems. The carriers should be non-toxic and stable in vivo, but interact with the target cells and release the loaded oligonucleotides intracellularly. We approached this challenge by developing a light-triggered liposomal delivery system for oligonucleotides based on a non-cationic and thermosensitive liposome with indocyanine green (ICG) as photosensitizer. The liposomes had efficient release properties, as 90% of the encapsulated oligonucleotides were released after 1-minute light exposure. Cell studies using an enhanced green fluorescent protein (EGFP)-based splicing assay with HeLa cells showed light-activated transfection with up to 70%-80% efficacy. Moreover, free ICG and oligonucleotides in solution transfected cells upon light induction with similar efficacy as the liposomal system. The light-triggered delivery induced moderate cytotoxicity (25%-35% reduction in cell viability) 1-2 days after transfection, but the cell growth returned to control levels in 4 days. In conclusion, the ICG-based light-triggered delivery is a promising method for oligonucleotides, and it can be used as a platform for further optimization and development.
Subject: oligonucleotide delivery
light-activated release
intracellular release
liposome
indocyanine green
IN-VIVO CHARACTERISTICS
INDOCYANINE GREEN
FLUORESCENCE PROPERTIES
LIPID NANOPARTICLES
SURFACE-CHARGE
LIPOSOMES
MECHANISM
VITRO
PHARMACOKINETICS
PENETRATION
317 Pharmacy
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