Characterization of polydactyly chondrocytes and their use in cartilage engineering

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Cavalli , E , Levinson , C , Hertl , M , Broguiere , N , Brück , O , Mustjoki , S , Gerstenberg , A , Weber , D , Salzmann , G , Steinwachs , M , Barreto , G & Zenobi-Wong , M 2019 , ' Characterization of polydactyly chondrocytes and their use in cartilage engineering ' , Scientific Reports , vol. 9 , no. 1 , 4275 . https://doi.org/10.1038/s41598-019-40575-w , https://doi.org/10.1038/s41598-019-40575-w

Title: Characterization of polydactyly chondrocytes and their use in cartilage engineering
Author: Cavalli, Emma; Levinson, Clara; Hertl, Matthias; Broguiere, Nicolas; Brück, Oscar; Mustjoki, Satu; Gerstenberg, Anja; Weber, Daniel; Salzmann, Gian; Steinwachs, Matthias; Barreto, Goncalo; Zenobi-Wong, Marcy
Contributor: University of Helsinki, Department of Clinical Chemistry and Hematology
University of Helsinki, Department of Clinical Chemistry and Hematology
University of Helsinki, ETH, Swiss Federal Institute of Technology Zurich
Date: 2019-03-12
Language: eng
Number of pages: 15
Belongs to series: Scientific Reports
ISSN: 2045-2322
URI: http://hdl.handle.net/10138/300632
Abstract: Treating cartilage injuries and degenerations represents an open surgical challenge. The recent advances in cell therapies have raised the need for a potent off-the-shelf cell source. Intra-articular injections of TGF-beta transduced polydactyly chondrocytes have been proposed as a chronic osteoarthritis treatment but despite promising results, the use of gene therapy still raises safety concerns. In this study, we characterized infant, polydactyly chondrocytes during in vitro expansion and chondrogenic redifferentiation. Polydactyly chondrocytes have a steady proliferative rate and re-differentiate in 3D pellet culture after up to five passages. Additionally, we demonstrated that polydactyly chondrocytes produce cartilage-like matrix in a hyaluronan-based hydrogel, namely transglutaminase cross-linked hyaluronic acid (HA-TG). We utilized the versatility of TG cross-linking to augment the hydrogels with heparin moieties. The heparin chains allowed us to load the scaffolds with TGF-beta 1 which induced cartilage-like matrix deposition both in vitro and in vivo in a subcutaneous mouse model. This strategy introduces the possibility to use infant, polydactyly chondrocytes for the clinical treatment of joint diseases.
Subject: EXPRESSING TRANSFORMING GROWTH-FACTOR-BETA-1
ARTICULAR-CARTILAGE
INFANT CHONDROCYTES
JOINT INJURY
GENE-THERAPY
REPAIR
BONE
PROTEIN
SAFETY
CELLS
3111 Biomedicine
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