Differential Spinal and Supraspinal Activation of Glia in a Rat Model of Morphine Tolerance

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Jokinen , V , Sidorova , Y , Viisanen , H , Suleymanova , I , Tiilikainen , H , Li , Z , Lilius , T O , Matlik , K , Anttila , J E , Airavaara , M , Tian , L , Rauhala , P V & Kalso , E A 2018 , ' Differential Spinal and Supraspinal Activation of Glia in a Rat Model of Morphine Tolerance ' , Neuroscience , vol. 375 , pp. 10-24 . https://doi.org/10.1016/j.neuroscience.2018.01.048

Title: Differential Spinal and Supraspinal Activation of Glia in a Rat Model of Morphine Tolerance
Author: Jokinen, Viljami; Sidorova, Yulia; Viisanen, Hanna; Suleymanova, Ilida; Tiilikainen, Henna; Li, Zhilin; Lilius, Tuomas O.; Matlik, Kert; Anttila, Jenni E.; Airavaara, Mikko; Tian, Li; Rauhala, Pekka V.; Kalso, Eija A.
Contributor: University of Helsinki, Medicum
University of Helsinki, Institute of Biotechnology
University of Helsinki, Medicum
University of Helsinki, Institute of Biotechnology
University of Helsinki, Department of Pharmacology
University of Helsinki, Research Programs Unit
University of Helsinki, Medicum
University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
University of Helsinki, Neuroscience Center
University of Helsinki, Medicum
University of Helsinki, Eija Kalso / Principal Investigator
Date: 2018-04-01
Language: eng
Number of pages: 15
Belongs to series: Neuroscience
ISSN: 0306-4522
URI: http://hdl.handle.net/10138/300672
Abstract: Development of tolerance is a well known pharmacological characteristic of opioids and a major clinical problem. In addition to the known neuronal mechanisms of opioid tolerance, activation of glia has emerged as a potentially significant new mechanism. We studied activation of microglia and astrocytes in morphine tolerance and opioid-induced hyperalgesia in rats using immunohistochemistry, flow cytometry and RNA sequencing in spinal-and supraspinal regions. Chronic morphine treatment that induced tolerance and hyperalgesia also increased immunoreactivity of spinal microglia in the dorsal and ventral horns. Flow cytometry demonstrated that morphine treatment increased the proportion of M2-polarized spinal microglia, but failed to impact the number or the proportion of M1-polarized microglia. In the transcriptome of microglial cells isolated from the spinal cord (SC), morphine treatment increased transcripts related to cell activation and defense response. In the studied brain regions, no activation of microglia or astrocytes was detected by immunohistochemistry, except for a decrease in the number of microglial cells in the substantia nigra. In flow cytometry, morphine caused a decrease in the number of microglial cells in the medulla, but otherwise no change was detected for the count or the proportion of M1-and M2-polarized microglia in the medulla or sensory cortex. No evidence for the activation of glia in the brain was seen. Our results suggest that glial activation associated with opioid tolerance and opioid-induced hyperalgesia occurs mainly at the spinal level. The transcriptome data suggest that the microglial activation pattern after chronic morphine treatment has similarities with that of neuropathic pain. (C) 2018 IBRO. Published by Elsevier Ltd. All rights reserved.
Subject: flow cytometry
opioid
neuroinflammation
nociception
microglia
transcriptomics
CENTRAL-NERVOUS-SYSTEM
GENE-RELATED PEPTIDE
NEUROPATHIC PAIN
ANTINOCICEPTIVE TOLERANCE
OPIOID ANALGESIA
CELL ACTIVATION
PARKINSONS-DISEASE
PROTEIN-ALPHA
CORD-INJURY
MICROGLIA
3112 Neurosciences
3124 Neurology and psychiatry
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