The effect of NETO2 on synaptosomal kainate receptor subunit levels in fear-related brain regions

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dc.contributor Helsingin yliopisto, Bio- ja ympäristötieteellinen tiedekunta, Bio- ja ympäristötieteellinen tiedekunta fi
dc.contributor University of Helsinki, Faculty of Biological and Environmental Sciences, Faculty of Biological and Environmental Sciences en
dc.contributor Helsingfors universitet, Bio- och miljövetenskapliga fakulteten, Bio- och miljövetenskapliga fakulteten sv
dc.contributor.author Rydgren, Emilie
dc.date.issued 2018
dc.identifier.uri URN:NBN:fi:hulib-201904041600
dc.identifier.uri http://hdl.handle.net/10138/300686
dc.description.abstract Kainate receptors (KARs) are glutamate receptors that modulate neurotransmission and neuronal excitability. They assemble from five subunits (GRIK1-5 or GluK1-5) present at both pre- and postsynaptic membranes. KAR function is regulated by neuropilin and tolloid-like (NETO) proteins, which also regulate postsynaptic GRIK2 abundance. Some KAR subunit gene variants associate with psychiatric disorders. Moreover, Grik1, Grik2 and Grik4 knock-out (KO) mice display changes in anxiety- and fear-related behaviours. In previous work, Neto2 KO mice expressed higher fear and impaired fear extinction in the fear conditioning paradigm. We hypothesised that this phenotype could be due to reduced KAR subunit abundance in fear-related brain regions, i.e. ventral hippocampus, amygdala and medial prefrontal cortex (mPFC). We specifically investigated GRIK2/3 and GRIK5 levels in the subcellular synaptosomal (SYN) fraction using western blot. We did not observe any difference between genotypes in any of the brain regions. However, our statistical power may have been insufficient, particularly for amygdala and mPFC. Also, an effect on synaptic KAR subunit abundance might be specific to either pre- or postsynaptic compartment, and thus more difficult to detect in SYN fractions. Alternatively, NETO2 absence may affect KAR actions instead of their subunit levels in fear-related brain regions, which could be examined through electrophysiological recordings. Ultimately, unravelling how a molecular system without NETO2 gives rise to fear behaviour in mice may lead to a better understanding of fear-related disorders in human and to new therapeutic strategies. en
dc.language.iso en
dc.publisher Helsingin yliopisto fi
dc.publisher University of Helsinki en
dc.publisher Helsingfors universitet sv
dc.subject Kainate receptor
dc.subject synapse
dc.subject NETO2
dc.subject psychiatric disorders
dc.subject anxiety
dc.subject fear conditioning
dc.subject cerebellum
dc.subject ventral hippocampus
dc.subject amygdala
dc.subject medial prefrontal cortex
dc.subject subcellular fractionation
dc.subject western blot
dc.title The effect of NETO2 on synaptosomal kainate receptor subunit levels in fear-related brain regions en
dc.title.alternative Effekten av NETO2 på nivån synaptosomala kainat receptor subenheter i rädslo-relaterade hjärnområden sv
dc.type.ontasot pro gradu -tutkielmat fi
dc.type.ontasot master's thesis en
dc.type.ontasot pro gradu-avhandlingar sv
dc.subject.discipline fysiologia ja neurotiede fi
dc.subject.discipline Physiology and Neuroscience en
dc.subject.discipline fysiologi och neurovetenskap sv
dct.identifier.urn URN:NBN:fi:hulib-201904041600

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