Tossavainen , M , Ilyass , U , Ollilainen , V , Valkonen , K , Ojala , A & Romantschuk , M 2019 , ' Influence of long term nitrogen limitation on lipid, protein and pigment production of Euglena gracilis in photoheterotrophic cultures ' , PeerJ , vol. 7 , 6624 . https://doi.org/10.7717/peerj.6624
Title: | Influence of long term nitrogen limitation on lipid, protein and pigment production of Euglena gracilis in photoheterotrophic cultures |
Author: | Tossavainen, Marika; Ilyass, Usman; Ollilainen, Velimatti; Valkonen, Kalle; Ojala, Anne; Romantschuk, Martin |
Contributor organization: | Environmental Sciences Department of Food and Nutrition Food quality and safety: lipids, vitamins and other bioactive compounds Food Sciences Ecosystems and Environment Research Programme Helsinki Institute of Sustainability Science (HELSUS) Viikki Plant Science Centre (ViPS) Anne Ojala / Principal Investigator Ecosystem processes (INAR Forest Sciences) Institute for Atmospheric and Earth System Research (INAR) Martin Romantschuk / Principal Investigator Aerovirology Research Group |
Date: | 2019-04-01 |
Language: | eng |
Number of pages: | 19 |
Belongs to series: | PeerJ |
ISSN: | 2167-8359 |
DOI: | https://doi.org/10.7717/peerj.6624 |
URI: | http://hdl.handle.net/10138/300796 |
Abstract: | Nitrogen limitation is considered a good strategy for enhancement of algal lipid production while conversely N repletion has been shown to result in biomass rich in proteins. In this study, the influence of long-term N limitation on Euglena gracilis fatty acid (FA), protein, chlorophyll a, and carotenoid concentrations was studied in N limited cultures. Biomass composition was analyzed from three-time points from N starved late stationary phase cultures, exposed to three different initial N concentrations in the growth medium. Total lipid content increased under N limitation in ageing cultures, but the low N content and prolonged cultivation time resulted in the formation of a high proportion of saturated FAs. Furthermore, growth as well as the production of proteins, chlorophyll a and carotenoids were enhanced in higher N concentrations and metabolism of these cellular components stayed stable during the stationary growth phase. Our findings showed that a higher N availability and a shorter cultivation time is a good strategy for efficient E. gracilis biomass production, regardless of whether the produced biomass is intended for maximal recovery of polyunsaturated FAs, proteins, or photosynthetic pigments. Additionally, we showed an increase of neoxanthin, beta-carotene, and diadinoxanthin as a response to higher N availability. |
Subject: |
Microalgae
Fatty acid EPA DHA LC-PUFA Carotenoid Carotene Neoxanthin Diadinoxanthin Nutrition FATTY-ACID-COMPOSITION MARINE MICROALGAE INTRACELLULAR NITROGEN CAROTENOIDS CONVERSION GROWTH ALGAE LIGHT ACCUMULATION CHLOROPHYLL 1182 Biochemistry, cell and molecular biology |
Peer reviewed: | Yes |
Rights: | cc_by |
Usage restriction: | openAccess |
Self-archived version: | publishedVersion |
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