Influence of long term nitrogen limitation on lipid, protein and pigment production of Euglena gracilis in photoheterotrophic cultures

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http://hdl.handle.net/10138/300796

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Tossavainen , M , Ilyass , U , Ollilainen , V , Valkonen , K , Ojala , A & Romantschuk , M 2019 , ' Influence of long term nitrogen limitation on lipid, protein and pigment production of Euglena gracilis in photoheterotrophic cultures ' , PeerJ , vol. 7 , 6624 . https://doi.org/10.7717/peerj.6624

Julkaisun nimi: Influence of long term nitrogen limitation on lipid, protein and pigment production of Euglena gracilis in photoheterotrophic cultures
Tekijä: Tossavainen, Marika; Ilyass, Usman; Ollilainen, Velimatti; Valkonen, Kalle; Ojala, Anne; Romantschuk, Martin
Tekijän organisaatio: Environmental Sciences
Department of Food and Nutrition
Food quality and safety: lipids, vitamins and other bioactive compounds
Food Sciences
Ecosystems and Environment Research Programme
Helsinki Institute of Sustainability Science (HELSUS)
Viikki Plant Science Centre (ViPS)
Anne Ojala / Principal Investigator
Ecosystem processes (INAR Forest Sciences)
Institute for Atmospheric and Earth System Research (INAR)
Martin Romantschuk / Principal Investigator
Aerovirology Research Group
Päiväys: 2019-04-01
Kieli: eng
Sivumäärä: 19
Kuuluu julkaisusarjaan: PeerJ
ISSN: 2167-8359
DOI-tunniste: https://doi.org/10.7717/peerj.6624
URI: http://hdl.handle.net/10138/300796
Tiivistelmä: Nitrogen limitation is considered a good strategy for enhancement of algal lipid production while conversely N repletion has been shown to result in biomass rich in proteins. In this study, the influence of long-term N limitation on Euglena gracilis fatty acid (FA), protein, chlorophyll a, and carotenoid concentrations was studied in N limited cultures. Biomass composition was analyzed from three-time points from N starved late stationary phase cultures, exposed to three different initial N concentrations in the growth medium. Total lipid content increased under N limitation in ageing cultures, but the low N content and prolonged cultivation time resulted in the formation of a high proportion of saturated FAs. Furthermore, growth as well as the production of proteins, chlorophyll a and carotenoids were enhanced in higher N concentrations and metabolism of these cellular components stayed stable during the stationary growth phase. Our findings showed that a higher N availability and a shorter cultivation time is a good strategy for efficient E. gracilis biomass production, regardless of whether the produced biomass is intended for maximal recovery of polyunsaturated FAs, proteins, or photosynthetic pigments. Additionally, we showed an increase of neoxanthin, beta-carotene, and diadinoxanthin as a response to higher N availability.
Avainsanat: Microalgae
Fatty acid
EPA
DHA
LC-PUFA
Carotenoid
Carotene
Neoxanthin
Diadinoxanthin
Nutrition
FATTY-ACID-COMPOSITION
MARINE MICROALGAE
INTRACELLULAR NITROGEN
CAROTENOIDS
CONVERSION
GROWTH
ALGAE
LIGHT
ACCUMULATION
CHLOROPHYLL
1182 Biochemistry, cell and molecular biology
Vertaisarvioitu: Kyllä
Tekijänoikeustiedot: cc_by
Pääsyrajoitteet: openAccess
Rinnakkaistallennettu versio: publishedVersion


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