Influence of long term nitrogen limitation on lipid, protein and pigment production of Euglena gracilis in photoheterotrophic cultures

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dc.contributor.author Tossavainen, Marika
dc.contributor.author Ilyass, Usman
dc.contributor.author Ollilainen, Velimatti
dc.contributor.author Valkonen, Kalle
dc.contributor.author Ojala, Anne
dc.contributor.author Romantschuk, Martin
dc.date.accessioned 2019-04-11T12:28:01Z
dc.date.available 2019-04-11T12:28:01Z
dc.date.issued 2019-04-01
dc.identifier.citation Tossavainen , M , Ilyass , U , Ollilainen , V , Valkonen , K , Ojala , A & Romantschuk , M 2019 , ' Influence of long term nitrogen limitation on lipid, protein and pigment production of Euglena gracilis in photoheterotrophic cultures ' , PeerJ , vol. 7 , 6624 . https://doi.org/10.7717/peerj.6624
dc.identifier.other PURE: 123879079
dc.identifier.other PURE UUID: 61ac542c-a41d-4a30-bdb5-47f9ded5852f
dc.identifier.other WOS: 000462855300002
dc.identifier.uri http://hdl.handle.net/10138/300796
dc.description.abstract Nitrogen limitation is considered a good strategy for enhancement of algal lipid production while conversely N repletion has been shown to result in biomass rich in proteins. In this study, the influence of long-term N limitation on Euglena gracilis fatty acid (FA), protein, chlorophyll a, and carotenoid concentrations was studied in N limited cultures. Biomass composition was analyzed from three-time points from N starved late stationary phase cultures, exposed to three different initial N concentrations in the growth medium. Total lipid content increased under N limitation in ageing cultures, but the low N content and prolonged cultivation time resulted in the formation of a high proportion of saturated FAs. Furthermore, growth as well as the production of proteins, chlorophyll a and carotenoids were enhanced in higher N concentrations and metabolism of these cellular components stayed stable during the stationary growth phase. Our findings showed that a higher N availability and a shorter cultivation time is a good strategy for efficient E. gracilis biomass production, regardless of whether the produced biomass is intended for maximal recovery of polyunsaturated FAs, proteins, or photosynthetic pigments. Additionally, we showed an increase of neoxanthin, beta-carotene, and diadinoxanthin as a response to higher N availability. en
dc.format.extent 19
dc.language.iso eng
dc.relation.ispartof PeerJ
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Microalgae
dc.subject Fatty acid
dc.subject EPA
dc.subject DHA
dc.subject LC-PUFA
dc.subject Carotenoid
dc.subject Carotene
dc.subject Neoxanthin
dc.subject Diadinoxanthin
dc.subject Nutrition
dc.subject FATTY-ACID-COMPOSITION
dc.subject MARINE MICROALGAE
dc.subject INTRACELLULAR NITROGEN
dc.subject CAROTENOIDS
dc.subject CONVERSION
dc.subject GROWTH
dc.subject ALGAE
dc.subject LIGHT
dc.subject ACCUMULATION
dc.subject CHLOROPHYLL
dc.subject 1182 Biochemistry, cell and molecular biology
dc.title Influence of long term nitrogen limitation on lipid, protein and pigment production of Euglena gracilis in photoheterotrophic cultures en
dc.type Article
dc.contributor.organization Environmental Sciences
dc.contributor.organization Department of Food and Nutrition
dc.contributor.organization Food quality and safety: lipids, vitamins and other bioactive compounds
dc.contributor.organization Food Sciences
dc.contributor.organization Ecosystems and Environment Research Programme
dc.contributor.organization Helsinki Institute of Sustainability Science (HELSUS)
dc.contributor.organization Viikki Plant Science Centre (ViPS)
dc.contributor.organization Anne Ojala / Principal Investigator
dc.contributor.organization Ecosystem processes (INAR Forest Sciences)
dc.contributor.organization Institute for Atmospheric and Earth System Research (INAR)
dc.contributor.organization Martin Romantschuk / Principal Investigator
dc.contributor.organization Aerovirology Research Group
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.7717/peerj.6624
dc.relation.issn 2167-8359
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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