Optimization of Early Steps in Oncolytic Adenovirus ONCOS-401 Production in T-175 and HYPERFlasks

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Kuryk , L , Moller , A-S W , Vuolanto , A , Pesonen , S , Garofalo , M , Cerullo , V & Jaderberg , M 2019 , ' Optimization of Early Steps in Oncolytic Adenovirus ONCOS-401 Production in T-175 and HYPERFlasks ' , International Journal of Molecular Sciences , vol. 20 , no. 3 , 621 . https://doi.org/10.3390/ijms20030621

Title: Optimization of Early Steps in Oncolytic Adenovirus ONCOS-401 Production in T-175 and HYPERFlasks
Author: Kuryk, Lukasz; Moller, Anne-Sophie W.; Vuolanto, Antti; Pesonen, Sari; Garofalo, Mariangela; Cerullo, Vincenzo; Jaderberg, Magnus
Other contributor: University of Helsinki, Faculty of Pharmacy
University of Helsinki, Division of Pharmaceutical Biosciences
University of Helsinki, Division of Pharmaceutical Biosciences



Date: 2019-02-01
Language: eng
Number of pages: 15
Belongs to series: International Journal of Molecular Sciences
ISSN: 1422-0067
DOI: https://doi.org/10.3390/ijms20030621
URI: http://hdl.handle.net/10138/300853
Abstract: Oncolytic adenoviruses can trigger lysis of tumor cells, induce an antitumor immune response, bypass classical chemotherapeutic resistance strategies of tumors, and provide opportunities for combination strategies. A major challenge is the development of scalable production methods for viral seed stocks and sufficient quantities of clinical grade viruses. Because of promising clinical signals in a compassionate use program (Advanced Therapy Access Program) which supported further development, we chose the oncolytic adenovirus ONCOS-401 as a testbed for a new approach to scale up. We found that the best viral production conditions in both T-175 flasks and HYPERFlasks included A549 cells grown to 220,000 cells/cm(2) (80% confluency), with ONCOS-401 infection at 30 multiplicity of infection (MOI), and an incubation period of 66 h. The Lysis A harvesting method with benzonase provided the highest viral yield from both T-175 and HYPERFlasks (10,887 +/- 100 and 14,559 +/- 802 infectious viral particles/cell, respectively). T-175 flasks and HYPERFlasks produced up to 2.1 x 10(9) +/- 0.2 and 1.75 x 10(9) +/- 0.08 infectious particles of ONCOS-401 per cm(2) of surface area, respectively. Our findings suggest a suitable stepwise process that can be applied to optimizing the initial production of other oncolytic viruses.
Subject: oncolytic adenovirus
CD40L
productivity
benzonase
manufacturing
optimization
cancer
MOI
harvesting time
virus productivity
TUMOR-SPECIFIC IMMUNITY
GENE-THERAPY
SOLID TUMORS
VIRUS
PURIFICATION
VECTORS
IMMUNOTHERAPY
BIOREACTOR
MELANOMA
RECEPTOR
116 Chemical sciences
1182 Biochemistry, cell and molecular biology
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