Optimization of Early Steps in Oncolytic Adenovirus ONCOS-401 Production in T-175 and HYPERFlasks

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dc.contributor.author Kuryk, Lukasz
dc.contributor.author Moller, Anne-Sophie W.
dc.contributor.author Vuolanto, Antti
dc.contributor.author Pesonen, Sari
dc.contributor.author Garofalo, Mariangela
dc.contributor.author Cerullo, Vincenzo
dc.contributor.author Jaderberg, Magnus
dc.date.accessioned 2019-04-12T08:57:01Z
dc.date.available 2019-04-12T08:57:01Z
dc.date.issued 2019-02-01
dc.identifier.citation Kuryk , L , Moller , A-S W , Vuolanto , A , Pesonen , S , Garofalo , M , Cerullo , V & Jaderberg , M 2019 , ' Optimization of Early Steps in Oncolytic Adenovirus ONCOS-401 Production in T-175 and HYPERFlasks ' , International Journal of Molecular Sciences , vol. 20 , no. 3 , 621 . https://doi.org/10.3390/ijms20030621
dc.identifier.other PURE: 123898947
dc.identifier.other PURE UUID: 0b68a310-1374-4c91-be37-832de82433dc
dc.identifier.other WOS: 000462412500165
dc.identifier.other Scopus: 85060915177
dc.identifier.uri http://hdl.handle.net/10138/300853
dc.description.abstract Oncolytic adenoviruses can trigger lysis of tumor cells, induce an antitumor immune response, bypass classical chemotherapeutic resistance strategies of tumors, and provide opportunities for combination strategies. A major challenge is the development of scalable production methods for viral seed stocks and sufficient quantities of clinical grade viruses. Because of promising clinical signals in a compassionate use program (Advanced Therapy Access Program) which supported further development, we chose the oncolytic adenovirus ONCOS-401 as a testbed for a new approach to scale up. We found that the best viral production conditions in both T-175 flasks and HYPERFlasks included A549 cells grown to 220,000 cells/cm(2) (80% confluency), with ONCOS-401 infection at 30 multiplicity of infection (MOI), and an incubation period of 66 h. The Lysis A harvesting method with benzonase provided the highest viral yield from both T-175 and HYPERFlasks (10,887 +/- 100 and 14,559 +/- 802 infectious viral particles/cell, respectively). T-175 flasks and HYPERFlasks produced up to 2.1 x 10(9) +/- 0.2 and 1.75 x 10(9) +/- 0.08 infectious particles of ONCOS-401 per cm(2) of surface area, respectively. Our findings suggest a suitable stepwise process that can be applied to optimizing the initial production of other oncolytic viruses. en
dc.format.extent 15
dc.language.iso eng
dc.relation.ispartof International Journal of Molecular Sciences
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject oncolytic adenovirus
dc.subject CD40L
dc.subject productivity
dc.subject benzonase
dc.subject manufacturing
dc.subject optimization
dc.subject cancer
dc.subject MOI
dc.subject harvesting time
dc.subject virus productivity
dc.subject GENE-THERAPY
dc.subject SOLID TUMORS
dc.subject VIRUS
dc.subject VECTORS
dc.subject BIOREACTOR
dc.subject MELANOMA
dc.subject RECEPTOR
dc.subject 116 Chemical sciences
dc.subject 1182 Biochemistry, cell and molecular biology
dc.title Optimization of Early Steps in Oncolytic Adenovirus ONCOS-401 Production in T-175 and HYPERFlasks en
dc.type Article
dc.contributor.organization Faculty of Pharmacy
dc.contributor.organization Drug Research Program
dc.contributor.organization ImmunoViroTherapy Lab
dc.contributor.organization Division of Pharmaceutical Biosciences
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.3390/ijms20030621
dc.relation.issn 1422-0067
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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