Dual RNA-seq analysis provides new insights into interactions between Norway spruce and necrotrophic pathogen Heterobasidion annosum s.l.

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Kovalchuk , A , Zeng , Z , Ghimire , R P , Kivimäenpää , M , Raffaello , T , Liu , M , Mukrimin , M , Kasanen , R A O , Sun , H , Julkunen-Tiitto , R , Holopainen , J K & Asiegbu , F O 2019 , ' Dual RNA-seq analysis provides new insights into interactions between Norway spruce and necrotrophic pathogen Heterobasidion annosum s.l. ' , BMC Plant Biology , vol. 19 , no. 2 , 2 . https://doi.org/10.1186/s12870-018-1602-0

Title: Dual RNA-seq analysis provides new insights into interactions between Norway spruce and necrotrophic pathogen Heterobasidion annosum s.l.
Author: Kovalchuk, Andriy; Zeng, Zhen; Ghimire, Rajendra P.; Kivimäenpää, Minna; Raffaello, Tommaso; Liu, Mengxia; Mukrimin, Mukrimin; Kasanen, Risto Aarne Olavi; Sun, Hui; Julkunen-Tiitto, Riitta; Holopainen, Jarmo K.; Asiegbu, Frederick Obioma
Contributor: University of Helsinki, Department of Forest Sciences
University of Helsinki, Department of Forest Sciences
University of Helsinki, Forest Ecology and Management
University of Helsinki, Department of Forest Sciences
University of Helsinki, Doctoral Programme in Plant Sciences
University of Helsinki, Department of Forest Sciences
University of Helsinki, Helsinki Institute of Sustainability Science (HELSUS)
Date: 2019-01-03
Language: eng
Number of pages: 17
Belongs to series: BMC Plant Biology
ISSN: 1471-2229
URI: http://hdl.handle.net/10138/300855
Abstract: BackgroundRoot and butt rot of conifer trees caused by fungi belonging to the Heterobasidion annosum species complex is one of the most economically important fungal diseases in commercial conifer plantations throughout the Northern hemisphere. We investigated the interactions between Heterobasidion fungi and their host by conducting dual RNA-seq and chemical analysis on Norway spruce trees naturally infected by Heterobasidion spp. We analyzed host and pathogen transcriptome and phenolic and terpenoid contents of the spruce trees.ResultsPresented results emphasize the role of the phenylpropanoid and flavonoid pathways in the chemical defense of Norway spruce trees. Accumulation of lignans was observed in trees displaying symptoms of wood decay. A number of candidate genes with a predicted role in the higher level regulation of spruce defense responses were identified. Our data indicate a possible role of abscisic acid (ABA) signaling in the spruce defense against Heterobasidion infection. Fungal transcripts corresponding to genes encoding carbohydrate- and lignin-degrading enzymes, secondary metabolism genes and effector-like genes were expressed during the host colonization.ConclusionsOur results provide additional insight into defense strategies employed by Norway spruce trees against Heterobasidion infection. The potential applications of the identified candidate genes as markers for higher resistance against root and butt rot deserve further evaluation.
Subject: ABSCISIC-ACID
CDNA CLONING
DEFENSE
DIFFERENTIAL EXPRESSION ANALYSIS
Dual RNA-seq
FUNCTIONAL-CHARACTERIZATION
Flavonoids
GENE-EXPRESSION
Heterobasidion
Norway spruce
PHENYLPROPANOID METABOLISM
PICEA-ABIES
Plant defense
Root and butt rot
TERPENE SYNTHASE
TRANSCRIPTION FACTOR
Transcriptomics
1183 Plant biology, microbiology, virology
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