Development and evaluation of a rapid nucleic acid amplification method to detect influenza A and B viruses in human respiratory specimens

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Elf , S , Auvinen , P , Jahn , L , Liikonen , K , Sjöblom , S , Saavalainen , P , Mäki , M & Eboigbodin , K E 2018 , ' Development and evaluation of a rapid nucleic acid amplification method to detect influenza A and B viruses in human respiratory specimens ' , Diagnostic Microbiology and Infectious Disease , vol. 92 , no. 1 , pp. 37-42 . https://doi.org/10.1016/j.diagmicrobio.2018.04.006

Title: Development and evaluation of a rapid nucleic acid amplification method to detect influenza A and B viruses in human respiratory specimens
Author: Elf, Sonja; Auvinen, Pauliina; Jahn, Lisa; Liikonen, Karoliina; Sjöblom, Solveig; Saavalainen, Päivi; Mäki, Minna; Eboigbodin, Kevin E.
Contributor: University of Helsinki, Research Programs Unit
Date: 2018-09
Language: eng
Number of pages: 6
Belongs to series: Diagnostic Microbiology and Infectious Disease
ISSN: 0732-8893
URI: http://hdl.handle.net/10138/300994
Abstract: Isothermal nucleic acid amplification methods can potentially shorten the amount of time required to diagnose influenza. We developed and evaluated a novel isothermal nucleic acid amplification method, RT-SIBA to rapidly detect and differentiate between influenza A and B viruses in a single reaction tube. The performance of the RT-SIBA Influenza assay was compared with two established RT-PCR methods. The sensitivities of the RT-SIBA, RealStar RT-PCR, and CDC RT-PCR assays for the detection of influenza A and B viruses in the clinical specimens were 98.8%, 100%, and 89.3%, respectively. All three assays demonstrated a specificity of 100%. The average time to positive result was significantly shorter with the RT-SIBA Influenza assay (90 min). The method can be run using battery-operated, portable devices with a small footprint and therefore has potential applications in both laboratory and near-patient settings. (C) 2018 Elsevier Inc. All rights reserved.
Subject: Influenza
Virus
Diagnostics
Isothermal
Amplification
Portable
INFECTIONS
CHALLENGES
DIAGNOSIS
3111 Biomedicine
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