Metabolic signature of extracellular vesicles depends on the cell culture conditions

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Palviainen , M , Saari , H , Kärkkäinen , O , Pekkinen , J , Auriola , S , Yliperttula , M , Puhka , M , Hanhineva , K & Siljander , P R-M 2019 , ' Metabolic signature of extracellular vesicles depends on the cell culture conditions ' , Journal of Extracellular Vesicles , vol. 8 , no. 1 , 1596669 .

Title: Metabolic signature of extracellular vesicles depends on the cell culture conditions
Author: Palviainen, Mari; Saari, Heikki; Kärkkäinen, Olli; Pekkinen, Jenna; Auriola, Seppo; Yliperttula, Marjo; Puhka, Maija; Hanhineva, Kati; Siljander, Pia R-M
Contributor organization: Extracellular Vesicles
Molecular and Integrative Biosciences Research Programme
Research Programs Unit
University of Helsinki
Faculty of Biological and Environmental Sciences
Division of Pharmaceutical Biosciences
Drug Research Program
Faculty of Pharmacy
Institute for Molecular Medicine Finland
Biopharmaceutics Group
Date: 2019-01-01
Language: eng
Number of pages: 12
Belongs to series: Journal of Extracellular Vesicles
ISSN: 2001-3078
Abstract: One of the greatest bottlenecks in extracellular vesicle (EV) research is the production of sufficient material in a consistent and effective way using in vitro cell models. Although the production of EVs in bioreactors maximizes EV yield in comparison to conventional cell cultures, the impact of their cell growth conditions on EVs has not yet been established. In this study, we grew two prostate cancer cell lines, PC-3 and VCaP, in conventional cell culture dishes and in two-chamber bioreactors to elucidate how the growth environment affects the EV characteristics. Specifically, we wanted to investigate the growth condition-dependent differences by non-targeted metabolite profiling using liquid chromatography-mass spectrometry (LC-MS) analysis. EVs were also characterized by their morphology, size distribution, and EV protein marker expression, and the EV yields were quantified by NTA. The use of bioreactor increased the EV yield >100 times compared to the conventional cell culture system. Regarding morphology, size distribution and surface markers, only minor differences were observed between the bioreactor-derived EVs (BR-EVs) and the EVs obtained from cells grown in conventional cell cultures (C-EVs). In contrast, metabolomic analysis revealed statistically significant differences in both polar and non-polar metabolites when the BR-EVs were compared to the C-EVs. The results show that the growth conditions markedly affected the EV metabolite profiles and that metabolomics was a sensitive tool to study molecular differences of EVs. We conclude that the cell culture conditions of EV production should be standardized and carefully detailed in publications and care should be taken when EVs from different production platforms are compared with each other for systemic effects.
Subject: Conditioned medium
cell culture
extracellular vesicles (EVs)
prostate cancer cell line
1182 Biochemistry, cell and molecular biology
Peer reviewed: Yes
Rights: cc_by_nc
Usage restriction: openAccess
Self-archived version: publishedVersion

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