Crystallization Kinetics of an Amorphous Pharmaceutical Compound Using Fluorescence-Lifetime-Imaging Microscopy

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dc.contributor.author Rautaniemi, Kaisa
dc.contributor.author Vuorimaa-Laukkanen, Elina
dc.contributor.author Strachan, Clare J.
dc.contributor.author Laaksonen, Timo
dc.date.accessioned 2019-04-24T11:52:02Z
dc.date.available 2019-04-24T11:52:02Z
dc.date.issued 2018-05
dc.identifier.citation Rautaniemi , K , Vuorimaa-Laukkanen , E , Strachan , C J & Laaksonen , T 2018 , ' Crystallization Kinetics of an Amorphous Pharmaceutical Compound Using Fluorescence-Lifetime-Imaging Microscopy ' , Molecular Pharmaceutics , vol. 15 , no. 5 , pp. 1964-1971 . https://doi.org/10.1021/acs.molpharmaceut.8b00117
dc.identifier.other PURE: 107120206
dc.identifier.other PURE UUID: a91e9419-cf16-4744-9844-54ef7ffe7bcb
dc.identifier.other WOS: 000431833700022
dc.identifier.other Scopus: 85046674658
dc.identifier.other ORCID: /0000-0002-6699-3004/work/45241207
dc.identifier.uri http://hdl.handle.net/10138/301179
dc.description.abstract Pharmaceutical scientists are increasingly interested in amorphous drug formulations especially because of their higher dissolution rates. Consequently, the thorough characterization and analysis of these formulations are becoming more and more important for the pharmaceutical industry. Here, fluorescence lifetime-imaging microscopy (FLIM) was used to monitor the crystallization of an amorphous pharmaceutical compound, indomethacin. Initially, we identified different solid indomethacin forms, amorphous and gamma- and alpha-crystalline, on the basis of their time-resolved fluorescence. All of the studied indomethacin forms showed biexponential decays with characteristic fluorescence lifetimes and amplitudes. Using this information, the crystallization of amorphous indomethacin upon storage in 60 degrees C was monitored for 10 days with FLIM. The progress of crystallization was detected as lifetime changes both in the FLIM images and in the fluorescence-decay curves extracted from the images. The fluorescence-lifetime amplitudes were used for quantitative analysis of the crystallization process. We also demonstrated that the fluorescence-lifetime distribution of the sample changed during crystallization, and when the sample was not moved between measuring times, the lifetime distribution could also be used for the analysis of the reaction kinetics. Our results clearly show that FLIM is a sensitive and nondestructive method for monitoring solid-state transformations on the surfaces of fluorescent samples. en
dc.format.extent 8
dc.language.iso eng
dc.relation.ispartof Molecular Pharmaceutics
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject fluorescence
dc.subject fluorescence lifetime
dc.subject amorphous materials
dc.subject crystal growth
dc.subject kinetics
dc.subject SOLID-STATE FLUORESCENCE
dc.subject INDOMETHACIN POLYMORPHS
dc.subject RAMAN-SPECTROSCOPY
dc.subject SURFACE CRYSTALLIZATION
dc.subject ACETYLSALICYLIC-ACID
dc.subject DISSOLUTION BEHAVIOR
dc.subject PHYSICAL STABILITY
dc.subject DOSAGE FORMS
dc.subject T-G
dc.subject DRUG
dc.subject 116 Chemical sciences
dc.subject 317 Pharmacy
dc.title Crystallization Kinetics of an Amorphous Pharmaceutical Compound Using Fluorescence-Lifetime-Imaging Microscopy en
dc.type Article
dc.contributor.organization Faculty of Pharmacy
dc.contributor.organization Clare Strachan / Research Group
dc.contributor.organization Division of Pharmaceutical Chemistry and Technology
dc.contributor.organization Formulation and industrial pharmacy
dc.contributor.organization Preclinical Drug Formulation and Analysis group
dc.contributor.organization Drug Research Program
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1021/acs.molpharmaceut.8b00117
dc.relation.issn 1543-8384
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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