In situ analysis of gelatinolytic activity in human dentin

Show full item record



Permalink

http://hdl.handle.net/10138/301438

Citation

Scarabello Stape , T H , Tjäderhane , L , Tezvergil-Mutluay , A , Da Silva , W G , dos Santos Silva , A R , da Silva , W J & Marques , M R 2018 , ' In situ analysis of gelatinolytic activity in human dentin ' , Acta histochemica : a journal of structural biochemistry, cell and tissue imaging , vol. 120 , no. 2 , pp. 136-141 . https://doi.org/10.1016/j.acthis.2017.12.008

Title: In situ analysis of gelatinolytic activity in human dentin
Author: Scarabello Stape, Thiago Henrique; Tjäderhane, Leo; Tezvergil-Mutluay, Arzu; Da Silva, Wagner Gomes; dos Santos Silva, Alan Roger; da Silva, Wander Jose; Marques, Marcelo Rocha
Contributor: University of Helsinki, Clinicum
Date: 2018
Language: eng
Number of pages: 6
Belongs to series: Acta histochemica : a journal of structural biochemistry, cell and tissue imaging
ISSN: 0065-1281
URI: http://hdl.handle.net/10138/301438
Abstract: Matrix metalloproteinases (MMPs) such as gelatinases are differentially expressed in human tissues. These enzymes cleave specific substrates involved in cell signaling, tissue development and remodeling and tissue breakdown. Recent evidences show that gelatinases are crucial for normal dentin development and their activity is maintained throughout the entire tooth function in the oral cavity. Due to the lack of information about the exact location and activity of gelatinases in mature human dentin, the present study was designed to examine gelatinolytic levels In sound dentin. In situ zymography using confocal microscopy was performed on both mineralized and demineralized dentin samples. Sites presenting gelatinase activity were identified throughout the entire biological tissue pursuing different gelatinolytic levels for distinct areas: predentin and dentinal tubule regions presented higher gelatinolytic activity compared to intertubular dentin. Dentin regions with higher gelatinolytic activity immunohistochemically were partially correlated with MMP-2 expression. The maintenance of gelatinolytic activity in mature dentin may have biological implications related to biomineralization of predentin and tubular/peritubular dentinal regions, as well as regulation of defensive mechanisms of the dentin-pulp complex.
Subject: in situ zymography
Matrix metalloproteinases
Immunohistochemistry
Enzymes
Tooth
HUMAN CORONAL DENTIN
MATRIX METALLOPROTEINASES
IMMUNOHISTOCHEMICAL LOCALIZATION
CYSTEINE CATHEPSINS
MECHANICAL-PROPERTIES
DISTINCT ROLES
SIALOPROTEIN
PROTEIN-1
VITRO
BIOMINERALIZATION
313 Dentistry
1182 Biochemistry, cell and molecular biology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
1_s2.0_S0065128117303513_main.pdf 794.1Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record