Transcription of lignocellulose-decomposition associated genes, enzyme activities and production of ethanol upon bioconversion of waste substrate by Phlebia radiata

Show full item record



Permalink

http://hdl.handle.net/10138/301440

Citation

Mäkinen , M A , Risulainen , N , Mattila , H K & Lundell , T K 2018 , ' Transcription of lignocellulose-decomposition associated genes, enzyme activities and production of ethanol upon bioconversion of waste substrate by Phlebia radiata ' , Applied Microbiology and Biotechnology , vol. 102 , no. 13 , pp. 5657-5672 . https://doi.org/10.1007/s00253-018-9045-y

Title: Transcription of lignocellulose-decomposition associated genes, enzyme activities and production of ethanol upon bioconversion of waste substrate by Phlebia radiata
Author: Mäkinen, Mari Annukka; Risulainen, Netta; Mattila, Hans Kristian; Lundell, Taina Kristina
Contributor: University of Helsinki, Department of Microbiology
University of Helsinki, Department of Microbiology
University of Helsinki, Department of Microbiology
Date: 2018-07
Language: eng
Number of pages: 16
Belongs to series: Applied Microbiology and Biotechnology
ISSN: 0175-7598
URI: http://hdl.handle.net/10138/301440
Abstract: Previously identified twelve plant cell wall degradation-associated genes of the white rot fungus Phlebia radiata were studied by RT-qPCR in semi-aerobic solid-state cultures on lignocellulose waste material, and on glucose-containing reference medium. Wood-decay-involved enzyme activities and ethanol production were followed to elucidate both the degradative and fermentative processes. On the waste lignocellulose substrate, P. radiata carbohydrate-active enzyme (CAZy) genes encoding cellulolytic and hemicellulolytic activities were significantly upregulated whereas genes involved in lignin modification displayed a more complex response. Two lignin peroxidase genes were differentially expressed on waste lignocellulose compared to glucose medium, whereas three manganese peroxidase-encoding genes were less affected. On the contrary, highly significant difference was noticed for three cellulolytic genes (cbhI_1, eg1, bgl1) with higher expression levels on the lignocellulose substrate than on glucose. This indicates expression of the wood-attacking degradative enzyme system by the fungus also on the recycled, waste core board material. During the second week of cultivation, ethanol production increased on the core board to 0.24 g/L, and extracellular activities against cellulose, xylan, and lignin were detected. Sugar release from the solid lignocellulose resulted with concomitant accumulation of ethanol as fermentation product. Our findings confirm that the fungus activates its white rot decay system also on industrially processed lignocellulose adopted as growth substrate, and under semi-aerobic cultivation conditions. Thus, P. radiata is a good candidate for lignocellulose-based renewable biotechnology to make biofuels and biocompounds from materials with less value for recycling or manufacturing.
Subject: 414 Agricultural biotechnology
Fungal biology
Genomics
Fungal metabolites
Wood decay fungi
Lignocellulose biodegradation
1183 Plant biology, microbiology, virology
Fungal biotechnology
Mycology
219 Environmental biotechnology
Waste bioconversion
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
Makinen_manuscript_revisedIII_unmarked_1.pdf 863.6Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record