Direct vertical transmission of ESBL/pAmpC-producing Escherichia coli limited in poultry production pyramid

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http://hdl.handle.net/10138/302573

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Oikarainen , P E , Pohjola , L K , Pietola , E S & Heikinheimo , A 2019 , ' Direct vertical transmission of ESBL/pAmpC-producing Escherichia coli limited in poultry production pyramid ' , Veterinary Microbiology , vol. 231 , pp. 100-106 . https://doi.org/10.1016/j.vetmic.2019.03.001

Title: Direct vertical transmission of ESBL/pAmpC-producing Escherichia coli limited in poultry production pyramid
Author: Oikarainen, Paula E.; Pohjola, Leena K.; Pietola, Eeva S.; Heikinheimo, Annamari
Contributor: University of Helsinki, Zoonotic Antimicrobial Resistance
University of Helsinki, Food Hygiene and Environmental Health
University of Helsinki, Zoonotic Antimicrobial Resistance
Date: 2019-04
Language: eng
Number of pages: 7
Belongs to series: Veterinary Microbiology
ISSN: 0378-1135
URI: http://hdl.handle.net/10138/302573
Abstract: Extended-spectrum beta-lactamase (ESBL) and plasmidic AmpC (pAmpC) producing Escherichia coli are found in the poultry production even without antibiotic use. The spread of these bacteria has been suggested to occur via imported parent birds, enabling transmission to production level broilers vertically via eggs. We studied transmission of ESBL/pAmpC-producing E. coli and E. coli without antibiotic selection by sampling imported parent birds (n = 450), egg surfaces prior to and after the incubation period (n = 300 and n = 428, respectively) and the laying house environment (n = 20). Samples were additionally taken from embryos (n = 422). To study the prevention of transmission, a competitive exclusion (CE) solution was added onto freshly laid eggs prior to incubation period (n = 150). Results showed carriage of ESBL/pAmpC-producing E. coil in parent birds (26.7%), the environment (5%) and egg surfaces before the incubation period (1.3%), but not from egg surfaces or embryos after the incubation period. Whole genome sequencing revealed ESBL/pAmpC-producing E. coil isolates belonging to clonal lineages ST429 and ST2040. However, the finding of E. coli cultured without antibiotic selection in two (2.2%) embryos strengthens the need to study E. coil transmission in poultry production in more depth. Since ESBL/pAmpC-producing E. coli seem not to persist on egg surfaces, there is no need to use CE solution ex ovo as a prevention method. The results indicate that other routes, such as for example transmission through fomites or horizontal gene transfer by other bacterial species, could be more important than vertical transmission in the spread of resistance in broiler production.
Subject: ESBL
pAmpC
Poultry production
Antimicrobial resistance
Whole genome sequencing
EXTENDED-SPECTRUM
ANTIMICROBIAL RESISTANCE
COMPETITIVE-EXCLUSION
EGGSHELL PENETRATION
E. COLI
PLASMIDS
SALMONELLA
BROILERS
BENCHMARKING
DYNAMICS
413 Veterinary science
1183 Plant biology, microbiology, virology
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