Bacterial biofilm in salivary stones

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http://hdl.handle.net/10138/302821

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Perez-Tanoira , R , Aarnisalo , A , Haapaniemi , A , Saarinen , R , Kuusela , P & Kinnari , T J 2019 , ' Bacterial biofilm in salivary stones ' , European Archives of Oto-Rhino-Laryngology , vol. 276 , no. 6 , pp. 1815-1822 . https://doi.org/10.1007/s00405-019-05445-1

Title: Bacterial biofilm in salivary stones
Author: Perez-Tanoira, Ramon; Aarnisalo, Antti; Haapaniemi, Aaro; Saarinen, Riitta; Kuusela, Pentti; Kinnari, Teemu J.
Contributor: University of Helsinki, Clinicum
University of Helsinki, Korva-, nenä- ja kurkkutautien klinikka
University of Helsinki, Department of Ophthalmology and Otorhinolaryngology
University of Helsinki, Korva-, nenä- ja kurkkutautien klinikka
University of Helsinki, Department of Bacteriology and Immunology
University of Helsinki, Korva-, nenä- ja kurkkutautien klinikka
Date: 2019-06
Language: eng
Number of pages: 8
Belongs to series: European Archives of Oto-Rhino-Laryngology
ISSN: 0937-4477
URI: http://hdl.handle.net/10138/302821
Abstract: PurposeTo assess the susceptibility of salivary stones to bacterial biofilm formation, which may be involved in the development of salivary gland infection, and to investigate a relation between microbiological aspects and patient characteristics.MethodsThis prospective study comprises of 54 patients with sialolithiasis attended in Helsinki University Hospital during 2014-2016. A total of 55 salivary stones were removed, and studied for biofilm formation using fluorescence microscopy and sonication. The isolated organisms were quantified and identified using matrix-assisted laser desorption ionization time-of-flight mass spectrometry.ResultsBiofilm formation was confirmed on the surface of 39 (70.9%) stones. A total of 96 microorganisms were isolated from 45 salivary stones (81.8%). Two or more organisms were isolated in 33 (73.3%) cases. The main isolates were Streptococcus mitis/oralis (n=27; 28.1%), followed by Streptococcusanginosus (n=10; 9.6%), Rothia spp. (n=8; 8.3%), Streptococcusconstellatus (n=7; 7.3%), and Streptococcusgordonii (n=6; 6.2%). In all patients showing pre-operative (12 cases) or peri-operative (three cases) drainage of pus, the presence of biofilm was detected in microscopy (p=0.004). Four patients showed post-operative infection, and in three of them (75.0%), the presence of biofilm was detected. Increased number of pus drainage was found among patients with reflux symptoms or use of proton-pump inhibitors.ConclusionsSalivary stones are susceptible to bacterial biofilm formation, which could be related with the development and severity of the inflammation and the refractory nature of the disease. Sonication of salivary gland stones could be a useful method for finding the etiology of the chronic infection.
Subject: Salivary stones
Biofilm
Sialadenitis
Sialolithiasis
Sialendoscopy
SIALOLITHIASIS
INFECTIONS
DIAGNOSIS
CULTURES
3125 Otorhinolaryngology, ophthalmology
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