Bacterial biofilm in salivary stones

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http://hdl.handle.net/10138/302821

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Perez-Tanoira , R , Aarnisalo , A , Haapaniemi , A , Saarinen , R , Kuusela , P & Kinnari , T J 2019 , ' Bacterial biofilm in salivary stones ' , European Archives of Oto-Rhino-Laryngology , vol. 276 , no. 6 , pp. 1815-1822 . https://doi.org/10.1007/s00405-019-05445-1

Title: Bacterial biofilm in salivary stones
Author: Perez-Tanoira, Ramon; Aarnisalo, Antti; Haapaniemi, Aaro; Saarinen, Riitta; Kuusela, Pentti; Kinnari, Teemu J.
Contributor organization: Clinicum
Department of Ophthalmology and Otorhinolaryngology
Korva-, nenä- ja kurkkutautien klinikka
HUS Head and Neck Center
Department of Bacteriology and Immunology
Medicum
Date: 2019-06
Language: eng
Number of pages: 8
Belongs to series: European Archives of Oto-Rhino-Laryngology
ISSN: 0937-4477
DOI: https://doi.org/10.1007/s00405-019-05445-1
URI: http://hdl.handle.net/10138/302821
Abstract: PurposeTo assess the susceptibility of salivary stones to bacterial biofilm formation, which may be involved in the development of salivary gland infection, and to investigate a relation between microbiological aspects and patient characteristics.MethodsThis prospective study comprises of 54 patients with sialolithiasis attended in Helsinki University Hospital during 2014-2016. A total of 55 salivary stones were removed, and studied for biofilm formation using fluorescence microscopy and sonication. The isolated organisms were quantified and identified using matrix-assisted laser desorption ionization time-of-flight mass spectrometry.ResultsBiofilm formation was confirmed on the surface of 39 (70.9%) stones. A total of 96 microorganisms were isolated from 45 salivary stones (81.8%). Two or more organisms were isolated in 33 (73.3%) cases. The main isolates were Streptococcus mitis/oralis (n=27; 28.1%), followed by Streptococcusanginosus (n=10; 9.6%), Rothia spp. (n=8; 8.3%), Streptococcusconstellatus (n=7; 7.3%), and Streptococcusgordonii (n=6; 6.2%). In all patients showing pre-operative (12 cases) or peri-operative (three cases) drainage of pus, the presence of biofilm was detected in microscopy (p=0.004). Four patients showed post-operative infection, and in three of them (75.0%), the presence of biofilm was detected. Increased number of pus drainage was found among patients with reflux symptoms or use of proton-pump inhibitors.ConclusionsSalivary stones are susceptible to bacterial biofilm formation, which could be related with the development and severity of the inflammation and the refractory nature of the disease. Sonication of salivary gland stones could be a useful method for finding the etiology of the chronic infection.
Subject: Salivary stones
Biofilm
Sialadenitis
Sialolithiasis
Sialendoscopy
SIALOLITHIASIS
INFECTIONS
DIAGNOSIS
CULTURES
3125 Otorhinolaryngology, ophthalmology
Peer reviewed: Yes
Rights: unspecified
Usage restriction: openAccess
Self-archived version: publishedVersion


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