Evaluation of the effect of experimentally induced cartilage defect and intra-articular hyaluronan on synovial fluid biomarkers in intercarpal joints of horses

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Niemela , T M , Tulamo , R-M , Uriel Carmona , J & Lopez , C 2019 , ' Evaluation of the effect of experimentally induced cartilage defect and intra-articular hyaluronan on synovial fluid biomarkers in intercarpal joints of horses ' , Acta Veterinaria Scandinavica , vol. 61 , 24 . https://doi.org/10.1186/s13028-019-0460-6

Title: Evaluation of the effect of experimentally induced cartilage defect and intra-articular hyaluronan on synovial fluid biomarkers in intercarpal joints of horses
Author: Niemela, Tytti Maaria; Tulamo, Riitta-Mari; Uriel Carmona, Jorge; Lopez, Catalina
Contributor: University of Helsinki, Equine and Small Animal Medicine
University of Helsinki, Equine and Small Animal Medicine
Date: 2019-05-30
Language: eng
Number of pages: 9
Belongs to series: Acta Veterinaria Scandinavica
ISSN: 0044-605X
URI: http://hdl.handle.net/10138/302830
Abstract: Background: Inflammatory and degenerative activity inside the joint can be studied in vivo by analysis of synovial fluid biomarkers. In addition to pro-inflammatory mediators, several anabolic and anti-inflammatory substances are produced during the disease process. They counteract the catabolic effects of the pro-inflammatory cytokines and thus diminish the cartilage damage. The response of synovial fluid biomarkers after intra-articular hyaluronan injection, alone or in combination with other substances, has been examined only in a few equine studies. The effects of hyaluronan on some pro-inflammatory mediators, such as prostaglandin E-2, have been documented but especially the effects on synovial fluid anti-inflammatory mediators are less studied. In animal models hyaluronan has been demonstrated to reduce pain via protecting nociceptive nerve endings and by blocking pain receptor channels. However, the results obtained for pain-relief of human osteoarthritis are contradictory. The aim of the study was to measure the synovial fluid IL-1ra, PDGF-BB, TGF-beta(1) and TNF-alpha concentrations before and after surgically induced cartilage defect, and following intra-articular hyaluronan injection in horses. Eight Standardbred horses underwent bilateral arthroscopic surgeries of their intercarpal joints under general anaesthesia, and cartilage defect was created on the dorsal edge of the third carpal bone of one randomly selected intercarpal joint of each horse. Five days post-surgery, one randomly selected intercarpal joint was injected intra-articular with 3 mL HA (20 mg/mL). Results: Operation type had no significant effect on the synovial fluid IL-1ra, PDGF-BB, TGF-beta(1) and TNF-alpha concentrations but compared with baseline, synovial fluid IL-1ra and TNF-alpha concentrations increased. Intra-articular hyaluronan had no significant effect on the biomarker concentrations but a trend of mild improvement in the clinical signs of intra-articular inflammation was seen. Conclusions: Creation of the cartilage defect and sham-operation lead to an increase of synovial fluid IL-1ra and TNF-alpha concentrations but changes in concentrations of anabolic growth factors TGF-beta(1) and PDGF-BB could not be documented 5 days after the arthroscopy. Intra-articular hyaluronan was well tolerated. Further research is needed to document possible treatment effects of intra-articular hyaluronan on the synovial fluid biomarkers of inflammation and cartilage metabolism.
Subject: Experimental study
Cartilage defect
Hyaluronan
Interleukin-1 receptor antagonist
Non-animal stabilized hyaluronic acid (NASHA)
Platelet-derived growth factor BB
Synovitis
Transforming growth factor beta(1)
Tumour necrosis factor alpha
TUMOR-NECROSIS-FACTOR
NF-KAPPA-B
GROWTH-FACTOR
TGF-BETA
GEL SUPERNATANTS
OSTEOARTHRITIS
CYTOKINE
EXPRESSION
ACID
RICH
413 Veterinary science
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