Distinct fatty acid signatures in infrapatellar fat pad and synovial fluid of patients with osteoarthritis versus rheumatoid arthritis

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http://hdl.handle.net/10138/303450

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Mustonen , A-M , Käkelä , R , Lehenkari , P , Huhtakangas , J , Turunen , S , Joukainen , A , Kaariainen , T , Paakkonen , T , Kroger , H & Nieminen , P 2019 , ' Distinct fatty acid signatures in infrapatellar fat pad and synovial fluid of patients with osteoarthritis versus rheumatoid arthritis ' , Arthritis research & therapy , vol. 21 , 124 . https://doi.org/10.1186/s13075-019-1914-y

Title: Distinct fatty acid signatures in infrapatellar fat pad and synovial fluid of patients with osteoarthritis versus rheumatoid arthritis
Author: Mustonen, Anne-Mari; Käkelä, Reijo; Lehenkari, Petri; Huhtakangas, Johanna; Turunen, Sanna; Joukainen, Antti; Kaariainen, Tommi; Paakkonen, Tommi; Kroger, Heikki; Nieminen, Petteri
Contributor: University of Helsinki, Molecular and Integrative Biosciences Research Programme
Date: 2019-05-22
Language: eng
Number of pages: 11
Belongs to series: Arthritis research & therapy
ISSN: 1478-6354
URI: http://hdl.handle.net/10138/303450
Abstract: Background: Infrapatellar fat pad (IFP) has recently emerged as a potential source of inflammation in knee arthropathies. It has been proposed to be one source of adipocytokines, fatty acids (FA), and FA-derived lipid mediators that could contribute to the pathophysiological processes in the knee joint. Alterations in synovial fluid (SF) lipid composition have been linked to both osteoarthritis (OA) and rheumatoid arthritis (RA). The aim of the present study was to compare the FA signatures in the IFP and SF of RA and OA patients. Methods: Pairs of IFP and SF samples were collected from the same knees of RA (n=10) and OA patients (n=10) undergoing total joint replacement surgery. Control SF samples (n=6) were harvested during diagnostic or therapeutic arthroscopic knee surgery unrelated to RA or OA. The FA composition in the total lipids of IFP and SF was determined by gas chromatography with flame ionization and mass spectrometric detection. Results: Arthropathies resulted in a significant reduction in the SF proportions of n-6 polyunsaturated FA (PUFA), more pronouncedly in OA than in RA. OA was also characterized with reduced percentages of 22:6n-3 and lower product/precursor ratios of n-3 PUFA. The proportions of total monounsaturated FA increased in both RA and OA SF. Regarding IFP, RA patients had lower proportions of 20:4n-6, total n-6 PUFA, and 22:6n-3, as well as lower product/precursor ratios of n-3 PUFA compared to OA patients. The average chain length of SF FA decreased in both diagnoses and the double bond index in OA. Conclusions: The observed complex alterations in the FA signatures could have both contributed to but also limited the inflammatory processes and cartilage destruction in the RA and OA knees.
Subject: Fatty acid
Infrapatellar fat pad
n-3 polyunsaturated fatty acids
n-6 polyunsaturated fatty acids
Osteoarthritis
Rheumatoid arthritis
Synovial fluid
KNEE OSTEOARTHRITIS
ARTICULAR-CARTILAGE
LIPID MEDIATORS
FISH-OIL
N-3
INFLAMMATION
ASSOCIATION
RATIO
3112 Neurosciences
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