The Water Extract of Juniperus communis L. Induces Cell Death and Sensitizes Cancer Cells to Cytostatic Drugs through p53 and PI3K/Akt Pathways

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Raasmaja , A , Stenius , U & Ghalali , A 2019 , ' The Water Extract of Juniperus communis L. Induces Cell Death and Sensitizes Cancer Cells to Cytostatic Drugs through p53 and PI3K/Akt Pathways ' , International Journal of Molecular Sciences , vol. 20 , no. 9 , 2054 . https://doi.org/10.3390/ijms20092054

Title: The Water Extract of Juniperus communis L. Induces Cell Death and Sensitizes Cancer Cells to Cytostatic Drugs through p53 and PI3K/Akt Pathways
Author: Raasmaja, Atso; Stenius, Ulla; Ghalali, Aram
Contributor: University of Helsinki, Division of Pharmacology and Pharmacotherapy
Date: 2019-05-01
Language: eng
Number of pages: 9
Belongs to series: International Journal of Molecular Sciences
ISSN: 1422-0067
URI: http://hdl.handle.net/10138/303498
Abstract: Juniper (Juniperus communis L.) is a northern coniferous plant generally used as a spice and for nutritional purposes in foods and drinks. It was previously reported that juniper extract (JE) affects p53 activity, cellular stress, and gene expression induced cell death in human neuroblastoma cells. Therefore, the effects of juniper on p53 and Akt signaling was examined further in A549 lung, 22RV1 and DU145 prostate, and HepG2 liver cancer cells using Western blot, confocal microscopy, and MTT analysis. We found that juniper simultaneously decreased cell viability, activated the p53 pathway, and inactivated the PI3K/Akt pathway. The p53 activation was associated with increased nuclear p53 level. Akt was dephosphorylated, and its inactivation was associated with increased levels of PHLPP1 and PHLPP2 phosphatases. Parallel increases of PARP suggest that JE decreased cell viability by activating cell death. In addition, JE potentiated the effects of gemcitabine and 5-fluorouracil anticancer drugs. Thus, JE can activate cell death in different cancer cell lines through p53 and Akt pathways.
Subject: cell death
plant extract
Juniperus communis L
p53
Akt
A549 non-small lung cancer cells
LUNG-CANCER
SURVIVAL
SH-SY5Y
NUCLEAR
1182 Biochemistry, cell and molecular biology
317 Pharmacy
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