A family AA5_2 carbohydrate oxidase from Penicillium rubens displays functional overlap across the AA5 family

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http://hdl.handle.net/10138/303508

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Mollerup , F , Aumala , V , Parikka , K M , Mathieu , Y , Brumer , H , Tenkanen , T M & Master , E 2019 , ' A family AA5_2 carbohydrate oxidase from Penicillium rubens displays functional overlap across the AA5 family ' , PLoS One , vol. 14 , no. 5 , 0216546 , pp. 1-19 . https://doi.org/10.1371/journal.pone.0216546

Title: A family AA5_2 carbohydrate oxidase from Penicillium rubens displays functional overlap across the AA5 family
Author: Mollerup, Filip; Aumala, Ville; Parikka, Kirsti Maria; Mathieu, Yan; Brumer, Harry; Tenkanen, Tiina Maija; Master, Emma
Other contributor: University of Helsinki, Department of Food and Nutrition
University of Helsinki, Department of Food and Nutrition

Date: 2019-05-15
Language: eng
Number of pages: 19
Belongs to series: PLoS One
ISSN: 1932-6203
DOI: https://doi.org/10.1371/journal.pone.0216546
URI: http://hdl.handle.net/10138/303508
Abstract: Copper radical alcohol oxidases belonging to auxiliary activity family 5, subfamily 2 (AA5_2) catalyze the oxidation of galactose and galactosides, as well as aliphatic alcohols. Despite their broad applied potential, so far very few AA5_2 members have been biochemically characterized. We report the recombinant production and biochemical characterization of an AA5_2 oxidase from Penicillium rubens Wisconsin 54-1255 (PruAA5_2A), which groups within an unmapped clade phylogenetically distant from those comprising AA5_2 members characterized to date. PruAA5_2 preferentially oxidized raffinose over galactose; however, its catalytic efficiency was 6.5 times higher on glycolaldehyde dimer compared to raffinose. Deep sequence analysis of characterized AA5_2 members highlighted amino acid pairs correlated to substrate range and conserved within the family. Moreover, PruAA5_2 activity spans substrate preferences previously reported for AA5 subfamily 1 and 2 members, identifying possible functional overlap across the AA5 family.
Subject: 414 Agricultural biotechnology
1183 Plant biology, microbiology, virology
GALACTOSE-OXIDASE
GLYOXAL OXIDASE
BINDING MODULE
ENZYME
EXPRESSION
MECHANISM
OXIDATION
AEROGELS
GENE
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