A preliminary transcriptome analysis suggests a transitory effect of vitamin D on mitochondrial function in obese young Finnish subjects

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Einarsdottir , E , Pekkinen , M , Krjutskov , K , Katayama , S , Kere , J , Mäkitie , O & Viljakainen , H 2019 , ' A preliminary transcriptome analysis suggests a transitory effect of vitamin D on mitochondrial function in obese young Finnish subjects ' , Endocrine Connections , vol. 8 , no. 5 , pp. 559-570 . https://doi.org/10.1530/EC-18-0537

Title: A preliminary transcriptome analysis suggests a transitory effect of vitamin D on mitochondrial function in obese young Finnish subjects
Author: Einarsdottir, Elisabet; Pekkinen, Minna; Krjutskov, Kaarel; Katayama, Shintaro; Kere, Juha; Mäkitie, Outi; Viljakainen, Heli
Contributor: University of Helsinki, Research Programme of Molecular Medicine
University of Helsinki, Clinicum
University of Helsinki, CAN-PRO - Translational Cancer Medicine Program
University of Helsinki, STEMM - Stem Cells and Metabolism Research Program
University of Helsinki, Lastentautien yksikkö
University of Helsinki, Department of Food and Nutrition
Date: 2019-05
Language: eng
Number of pages: 12
Belongs to series: Endocrine Connections
ISSN: 2049-3614
URI: http://hdl.handle.net/10138/303543
Abstract: Objective: The effect of vitamin D at the transcriptome level is poorly understood, and furthermore, it is unclear if it differs between obese and normal-weight subjects. The objective of the study was to explore the transcriptome effects of vitamin D supplementation. Design and methods: We analysed peripheral blood gene expression using GlobinLock oligonucleotides followed by RNA sequencing in individuals participating in a 12-week randomised double-blinded placebo-controlled vitamin D intervention study. The study involved 18 obese and 18 normal-weight subjects (of which 20 males) with mean (+/- s.D.) age 20.4 (+/- 2.5) years and BMIs 36 (+/- 10) and 23 (+/- 4) kg/m(2), respectively. The supplemental daily vitamin D dose was 50 mu g (2000 IU). Data were available at baseline, 6- and 12-week time points and comparisons were performed between the vitamin D and placebo groups separately in obese and normal-weight subjects. Results: Significant transcriptomic changes were observed at 6 weeks, and only in the obese subjects: 1724 genes were significantly upregulated and 186 genes were downregulated in the vitamin D group compared with placebo. Further analyses showed several enriched gene categories connected to mitochondrial function and metabolism, and the most significantly enriched pathway was related to oxidative phosphorylation (adjusted P value 3.08 x 10(-14)). Taken together, our data suggest an effect of vitamin D supplementation on mitochondrial function in obese subjects. Conclusions: Vitamin D supplementation affects gene expression in obese, but not in normal-weight subjects. The altered genes are enriched in pathways related to mitochondrial function. The present study increases the understanding of the effects of vitamin D at the transcriptome level.
Subject: vitamin D
gene expression
obesity
transcriptome
mitochondrial function
intervention
SINGLE-CELL TRANSCRIPTOME
D DEFICIENCY
D-RECEPTOR
D SUPPLEMENTATION
SERUM 25-HYDROXYCHOLECALCIFEROL
INSULIN-RESISTANCE
EXPRESSION
CHILDREN
DISEASE
WEIGHT
3111 Biomedicine
3121 General medicine, internal medicine and other clinical medicine
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