Cerebral Dopamine Neurotrophic Factor Diffuses Around the Brainstem and Does Not Undergo Anterograde Transport After Injection to the Substantia Nigra

Show full item record



Permalink

http://hdl.handle.net/10138/303562

Citation

Albert , K , Renko , J-M , Mätlik , K , Airavaara , M & Voutilainen , M H 2019 , ' Cerebral Dopamine Neurotrophic Factor Diffuses Around the Brainstem and Does Not Undergo Anterograde Transport After Injection to the Substantia Nigra ' , Frontiers in Neuroscience , vol. 13 , 590 . https://doi.org/10.3389/fnins.2019.00590

Title: Cerebral Dopamine Neurotrophic Factor Diffuses Around the Brainstem and Does Not Undergo Anterograde Transport After Injection to the Substantia Nigra
Author: Albert, Katrina; Renko, Juho-Matti; Mätlik, Kert; Airavaara, Mikko; Voutilainen, Merja H.
Other contributor: University of Helsinki, Institute of Biotechnology
University of Helsinki, Regenerative pharmacology group
University of Helsinki, Institute of Biotechnology
University of Helsinki, Neuroscience Center
University of Helsinki, Regenerative Neuroscience





Date: 2019-06-11
Language: eng
Number of pages: 8
Belongs to series: Frontiers in Neuroscience
ISSN: 1662-453X
DOI: https://doi.org/10.3389/fnins.2019.00590
URI: http://hdl.handle.net/10138/303562
Abstract: Cerebral dopamine neurotrophic factor (CDNF) has shown therapeutic potential in rodent and non-human primate models of Parkinson's disease by protecting the dopamine neurons from degeneration and even restoring their phenotype and function. Previously, neurorestorative efficacy of CDNF in the 6-hydroxydopamine (6-OHDA) model of Parkinson's disease as well as diffusion of the protein in the striatum (STR) has been demonstrated and studied. Here, experiments were performed to characterize the diffusion and transport of supra-nigral CDNF in non-lesioned rats. We injected recombinant human CDNF to the substantia nigra (SN) of naive male Wistar rats and analyzed the brains 2, 6, and 24 h after injections. We performed immunohistochemical stainings using an antibody specific to human CDNF and radioactivity measurements after injecting iodinated CDNF. Unlike the previously reported striatonigral retrograde transport seen after striatal injection, active anterograde transport of CDNF to the STR could not be detected after nigral injection. There was, however, clear diffusion of CDNF to the brain areas surrounding the SN, and CDNF colocalized with tyrosine hydroxylase (TH)-positive neurons. Overall, our results provide insight on how CDNF injected to the SN may act in this region of the brain.
Subject: 3112 Neurosciences
cerebral dopamine neurotrophic factor
substantia nigra
Parkinson's disease
striatum
diffusion
transport
SUBTHALAMIC NUCLEUS
CDNF PROTECTS
BASAL GANGLIA
NEURONS
PARVALBUMIN
SYSTEM
FAMILY
RAT
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
fnins_13_00590.pdf 2.442Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record