Exploring Light-Sensitive Nanocarriers for Simultaneous Triggered Antibiotic Release and Disruption of Biofilms Upon Generation of Laser-Induced Vapor Nanobubbles

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Teirlinck , E , Barras , A , Liu , J , Fraire , J C , Lajunen , T , Xiong , R , Forier , K , Li , C , Urtti , A , Boukherroub , R , Szunerits , S , De Smedt , S C , Coenye , T & Braeckmans , K 2019 , ' Exploring Light-Sensitive Nanocarriers for Simultaneous Triggered Antibiotic Release and Disruption of Biofilms Upon Generation of Laser-Induced Vapor Nanobubbles ' , Pharmaceutics , vol. 11 , no. 5 , 201 . https://doi.org/10.3390/pharmaceutics11050201

Title: Exploring Light-Sensitive Nanocarriers for Simultaneous Triggered Antibiotic Release and Disruption of Biofilms Upon Generation of Laser-Induced Vapor Nanobubbles
Author: Teirlinck, Eline; Barras, Alexandre; Liu, Jing; Fraire, Juan C.; Lajunen, Tatu; Xiong, Ranhua; Forier, Katrien; Li, Chengnan; Urtti, Arto; Boukherroub, Rabah; Szunerits, Sabine; De Smedt, Stefaan C.; Coenye, Tom; Braeckmans, Kevin
Contributor: University of Helsinki, Division of Pharmaceutical Biosciences
University of Helsinki, Drug Research Program
Date: 2019-05
Language: eng
Number of pages: 15
Belongs to series: Pharmaceutics
ISSN: 1999-4923
URI: http://hdl.handle.net/10138/303565
Abstract: Impaired penetration of antibiotics through bacterial biofilms is one of the reasons for failure of antimicrobial therapy. Hindered drug diffusion is caused on the one hand by interactions with the sticky biofilm matrix and on the other hand by the fact that bacterial cells are organized in densely packed clusters of cells. Binding interactions with the biofilm matrix can be avoided by encapsulating the antibiotics into nanocarriers, while interfering with the integrity of the dense cell clusters can enhance drug transport deep into the biofilm. Vapor nanobubbles (VNB), generated from laser irradiated nanoparticles, are a recently reported effective way to loosen up the biofilm structure in order to enhance drug transport and efficacy. In the present study, we explored if the disruptive force of VNB can be used simultaneously to interfere with the biofilm structure and trigger antibiotic release from light-responsive nanocarriers. The antibiotic tobramycin was incorporated in two types of light-responsive nanocarriersliposomes functionalized with gold nanoparticles (Lip-AuNP) and graphene quantum dots (GQD)and their efficacy was evaluated on Pseudomonas aeruginosa biofilms. Even though the anti-biofilm efficacy of tobramycin was improved by liposomal encapsulation, electrostatic functionalization with 70 nm AuNP unfortunately resulted in premature leakage of tobramycin in a matter of hours. Laser-irradiation consequently did not further improve P. aeruginosa biofilm eradication. Adsorption of tobramycin to GQD, on the other hand, did result in a stable formulation with high encapsulation efficiency, without burst release of tobramycin from the nanocarriers. However, even though laser-induced VNB formation from GQD resulted in biofilm disruption, an enhanced anti-biofilm effect was not achieved due to tobramycin not being efficiently released from GQD. Even though this study was unsuccessful in designing suitable nanocarriers for simultaneous biofilm disruption and light-triggered release of tobramycin, it provides insights into the difficulties and challenges that need to be considered for future developments in this regard.
Subject: vapor nanobubbles
laser treatment
triggered release
liposomes
gold nanoparticles
graphene quantum dots
biofilms
diffusion barrier
DEHYDRATION-REHYDRATION VESICLES
REDUCED GRAPHENE OXIDE
DRUG-DELIVERY
BACTERIAL
NANOPARTICLES
PENETRATION
INSULIN
317 Pharmacy
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