Enzymatic analysis of levan produced by lactic acid bacteria in fermented doughs

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Shi , Q , Hou , Y , Yan , X , Mørkeberg , K B R & Tenkanen , T M 2019 , ' Enzymatic analysis of levan produced by lactic acid bacteria in fermented doughs ' , Carbohydrate Polymers , vol. 208 , no. s , pp. 285-293 . https://doi.org/10.1016/j.carbpol.2018.12.044

Title: Enzymatic analysis of levan produced by lactic acid bacteria in fermented doughs
Author: Shi, Qiao; Hou, Yaxi; Yan, Xu; Mørkeberg , Kristian B.R.; Tenkanen, Tiina Maija
Contributor organization: Department of Food and Nutrition
Date: 2019-03-15
Language: eng
Number of pages: 9
Belongs to series: Carbohydrate Polymers
ISSN: 0144-8617
DOI: https://doi.org/10.1016/j.carbpol.2018.12.044
URI: http://hdl.handle.net/10138/303567
Abstract: Levans and inulins are fructans with mainly beta-(2 -> 6) and beta-(2 -> 1) linkages, respectively. Levans are produced by many lactic acid bacteria, e.g. during sourdough fermentation. Levans have shown prebiotic properties and may also function as in situ-produced hydrocolloids. So far, levan contents have been measured by acid hydrolysis, which cannot distinguish levans from e.g. inulins. In order to develop a specific analysis for levan in food matrices, a Paenibacillus amylolyticus endolevanase was combined with exoinulinase for levan hydrolysis. A separate endoinulinase treatment was used to detect the possible presence of inulin. Interfering sugars were removed by a pre-wash with aqueous ethanol. Levan content was estimated from fructose and glucose released in the hydrolysis, with a correction made for the residual fructose and glucose-containing sugars. The method was validated using wheat model doughs spiked with commercial Erwinia levan, and tested by analyzing levan content in Leuconostoc mesenteroides DSM 20343-fermented fava bean doughs.
Subject: 416 Food Science
Levan
lactic acid bacterium
Exopolysaccharide
fructan
Levanase
Quantification
LACTOBACILLUS-REUTERI
WEISSELLA-CIBARIA
INULIN
Levansucrase
SUCROSE
FRUCTOSYLTRANSFERASE
POLYSACCHARIDES
INULOSUCRASE
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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