DNA methylation changes in inflammation-related genes in ulcerative colitis-associated colorectal cancer

Visa fullständig post



Permalänk

http://urn.fi/URN:NBN:fi:hulib-201906283088
Titel: DNA methylation changes in inflammation-related genes in ulcerative colitis-associated colorectal cancer
Författare: Ukwattage, Sanjeevi
Medarbetare: Helsingin yliopisto, Bio- ja ympäristötieteellinen tiedekunta
University of Helsinki, Faculty of Biological and Environmental Sciences
Helsingfors universitet, Bio- och miljövetenskapliga fakulteten
Utgivare: Helsingin yliopisto
Datum: 2019
Språk: eng
Permanenta länken (URI): http://urn.fi/URN:NBN:fi:hulib-201906283088
http://hdl.handle.net/10138/303614
Nivå: pro gradu-avhandlingar
Utbildningsprogram: Genetiikan ja molekulaaristen biotieteiden maisteriohjelma
Master's Programme in Genetics and Molecular Biosciences
Magisterprogrammet i genetik och molekylära biovetenskaper
Studieinriktning: Genetiikan ja genomiikan opintosuunta
Genetics and Genomics
Genetik och genomik
Abstrakt: Background- Colorectal cancer (CRC) is the third most common epithelial carcinoma. There is an increased risk of colorectal cancer in people with longstanding inflammation in the large intestine, including individuals with ulcerative colitis (UC). Epigenetic changes in CRC such as aberrant DNA methylation alterations are common changes in human cancer. The aim of this study is to identify the DNA methylation alterations of selected inflammation related genes in UC-CRC vs. Lynch syndrome (LS). Method- DNA was extracted from archival tissue specimens from normal and tumor samples from UC-CRC (n= 31), and LS-CRC (n=29). Methylation-specific multiple ligation-dependent probe amplification (MS-MLPA) assays were used to detect CIMP status and CpG promoter methylation status of seven inflammation related genes. Microsatellite instability analysis was carried out using two mononucleotide repeat markers BAT25 and BAT26. Results- Increased hypermethylation frequencies in carcinoma vs. normal colonic mucosa were detected for all the inflammatory marker genes in specimens of UC-CRC patients. Statistically significant differences for methylation frequencies were observed in the NTSR1 gene (p value =0.008) and SOCS2 gene (p value =0.04) in specimens of UC-CRC patients. NTSR1 gene showed significantly increased methylation of normal colonic mucosae from UC-CRC vs. LS patients (p value=0.01). Conclusion- UC-CRC and LS tumor specimens revealed varying frequencies of hypermethylation in all the inflammatory genes. Methylation of the NTSR1 in the normal colonic mucosa suggests a possible field defect in UC-CRC, and could thus be used as an early biomarker to detect increased UC-CRC risk in non-neoplastic epithelium.
Subject: Colorectal cancer
Ulcerative Colitis
DNA methylation
Inflammation


Filer under denna titel

Totalt antal nerladdningar: Laddar...

Filer Storlek Format Granska
Ukwattage_Sanjeevi_pro_gradu_2019.pdf 7.867Kb PDF Granska/Öppna

Detta dokument registreras i samling:

Visa fullständig post