Identification and structural analysis of cereal arabinoxylan-derived oligosaccharides by negative ionization HILIC-MS/MS

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http://hdl.handle.net/10138/303617

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Juvonen , M K , Kotiranta , M , Jokela , J K , Tuomainen , P & Tenkanen , T M 2019 , ' Identification and structural analysis of cereal arabinoxylan-derived oligosaccharides by negative ionization HILIC-MS/MS ' , Food Chemistry , vol. 275 , pp. 176-185 . https://doi.org/10.1016/j.foodchem.2018.09.074

Title: Identification and structural analysis of cereal arabinoxylan-derived oligosaccharides by negative ionization HILIC-MS/MS
Author: Juvonen, Minna Katriina; Kotiranta, Markus; Jokela, Jouni Kalevi; Tuomainen, Päivi; Tenkanen, Tiina Maija
Contributor: University of Helsinki, Department of Food and Nutrition
University of Helsinki, Department of Food and Nutrition
University of Helsinki, Department of Microbiology
University of Helsinki, Department of Food and Nutrition
University of Helsinki, Department of Food and Nutrition
Date: 2019-03-01
Number of pages: 10
Belongs to series: Food Chemistry
ISSN: 0308-8146
URI: http://hdl.handle.net/10138/303617
Abstract: Recent works provide evidence of the prebiotic potential of arabinoxylan-derived oligosaccharides (A)XOS. In this study, we developed a structural analysis for cereal-derived (A)XOS by negative ionization HILIC-MS/MS. Initially, we assessed twelve (A)XOS samples of known structures with different linkage positions and branching points by direct-infusion negative ESI-MSn. We subsequently developed the negative ion HILIC-MS/MS with a post-column addition of ammonium chloride. The selected (A)XOS represented both linear (arabinofuranosyl residue linked to the non-reducing end of xylooligosaccharide) and branched structures. Each (A)XOS sample produced a specific spectrum in negative ion ESI-MSn. By analyzing cross-ring fragment ions, we determined the linkage positions of linear (A)XOS. The presence or absence of diagnostic ions in the MS3 allowed us to detect different branches (O-2- or/and O-3-linked arabinofuranosyl with/or without O-4-linked xylopyranosyl at the non-reducing end). Furthermore, we could identify all analyzed samples by HILIC-MS/MS, based on the formed spectral library and chromatographic retention times.
Subject: 416 Food Science
Tandem mass spectrometry
Oligosaccharides
Arabinoxylan
Negative ionization
Linkage analysis
ELECTROSPRAY MASS-SPECTROMETRY
ASSISTED-LASER-DESORPTION/IONIZATION
HUMAN-MILK OLIGOSACCHARIDES
UNDERIVATIZED OLIGOSACCHARIDES
LIQUID-CHROMATOGRAPHY
XYLO-OLIGOSACCHARIDES
ION MODE
PART 1
SPECTRA
MSN
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