Identification and structural analysis of cereal arabinoxylan-derived oligosaccharides by negative ionization HILIC-MS/MS
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Juvonen , M K , Kotiranta , M , Jokela , J K , Tuomainen , P & Tenkanen , T M 2019 , ' Identification and structural analysis of cereal arabinoxylan-derived oligosaccharides by negative ionization HILIC-MS/MS ' , Food Chemistry , vol. 275 , pp. 176-185 . https://doi.org/10.1016/j.foodchem.2018.09.074
| Title: | Identification and structural analysis of cereal arabinoxylan-derived oligosaccharides by negative ionization HILIC-MS/MS |
| Author: | Juvonen, Minna Katriina; Kotiranta, Markus; Jokela, Jouni Kalevi; Tuomainen, Päivi; Tenkanen, Tiina Maija |
| Contributor: | University of Helsinki, Department of Food and Nutrition University of Helsinki, Department of Food and Nutrition University of Helsinki, Department of Microbiology University of Helsinki, Department of Food and Nutrition University of Helsinki, Department of Food and Nutrition |
| Date: | 2019-03-01 |
| Number of pages: | 10 |
| Belongs to series: | Food Chemistry |
| ISSN: | 0308-8146 |
| URI: | http://hdl.handle.net/10138/303617 |
| Abstract: | Recent works provide evidence of the prebiotic potential of arabinoxylan-derived oligosaccharides (A)XOS. In this study, we developed a structural analysis for cereal-derived (A)XOS by negative ionization HILIC-MS/MS. Initially, we assessed twelve (A)XOS samples of known structures with different linkage positions and branching points by direct-infusion negative ESI-MSn. We subsequently developed the negative ion HILIC-MS/MS with a post-column addition of ammonium chloride. The selected (A)XOS represented both linear (arabinofuranosyl residue linked to the non-reducing end of xylooligosaccharide) and branched structures. Each (A)XOS sample produced a specific spectrum in negative ion ESI-MSn. By analyzing cross-ring fragment ions, we determined the linkage positions of linear (A)XOS. The presence or absence of diagnostic ions in the MS3 allowed us to detect different branches (O-2- or/and O-3-linked arabinofuranosyl with/or without O-4-linked xylopyranosyl at the non-reducing end). Furthermore, we could identify all analyzed samples by HILIC-MS/MS, based on the formed spectral library and chromatographic retention times. |
| Subject: |
416 Food Science
Tandem mass spectrometry Oligosaccharides Arabinoxylan Negative ionization Linkage analysis ELECTROSPRAY MASS-SPECTROMETRY ASSISTED-LASER-DESORPTION/IONIZATION HUMAN-MILK OLIGOSACCHARIDES UNDERIVATIZED OLIGOSACCHARIDES LIQUID-CHROMATOGRAPHY XYLO-OLIGOSACCHARIDES ION MODE PART 1 SPECTRA MSN |
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