CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response

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http://hdl.handle.net/10138/303675

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Haapaniemi , E , Botla , S , Persson , J , Schmierer , B & Taipale , J 2018 , ' CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response ' , Nature Medicine , vol. 24 , no. 7 , pp. 927-+ . https://doi.org/10.1038/s41591-018-0049-z

Title: CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response
Author: Haapaniemi, Emma; Botla, Sandeep; Persson, Jenna; Schmierer, Bernhard; Taipale, Jussi
Contributor: University of Helsinki, Genome-Scale Biology (GSB) Research Program
University of Helsinki, Doctoral Programme in Integrative Life Science
Date: 2018-07
Language: eng
Number of pages: 6
Belongs to series: Nature Medicine
ISSN: 1078-8956
URI: http://hdl.handle.net/10138/303675
Abstract: Here, we report that genome editing by CRISPR-Cas9 induces a p53-mediated DNA damage response and cell cycle arrest in immortalized human retinal pigment epithelial cells, leading to a selection against cells with a functional p53 pathway. Inhibition of p53 prevents the damage response and increases the rate of homologous recombination from a donor template. These results suggest that p53 inhibition may improve the efficiency of genome editing of untransformed cells and that p53 function should be monitored when developing cell-based therapies utilizing CRISPR-Cas9.
Subject: CELLS
INHIBITION
EFFICIENCY
REPAIR
GENES
3111 Biomedicine
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