CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response

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dc.contributor University of Helsinki, Genome-Scale Biology (GSB) Research Program en
dc.contributor University of Helsinki, Doctoral Programme in Integrative Life Science en Haapaniemi, Emma Botla, Sandeep Persson, Jenna Schmierer, Bernhard Taipale, Jussi 2019-06-30T21:46:16Z 2021-05-08T21:46:18Z 2018-07
dc.identifier.citation Haapaniemi , E , Botla , S , Persson , J , Schmierer , B & Taipale , J 2018 , ' CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response ' , Nature Medicine , vol. 24 , no. 7 , pp. 927-+ . en
dc.identifier.issn 1078-8956
dc.identifier.other PURE: 114801223
dc.identifier.other PURE UUID: 8f67cc84-e64b-4e1c-84f3-f8fca5cc0ae0
dc.identifier.other WOS: 000438187700019
dc.identifier.other Scopus: 85048349385
dc.description.abstract Here, we report that genome editing by CRISPR-Cas9 induces a p53-mediated DNA damage response and cell cycle arrest in immortalized human retinal pigment epithelial cells, leading to a selection against cells with a functional p53 pathway. Inhibition of p53 prevents the damage response and increases the rate of homologous recombination from a donor template. These results suggest that p53 inhibition may improve the efficiency of genome editing of untransformed cells and that p53 function should be monitored when developing cell-based therapies utilizing CRISPR-Cas9. en
dc.format.extent 6
dc.language.iso eng
dc.relation.ispartof Nature Medicine
dc.rights en
dc.subject CELLS en
dc.subject INHIBITION en
dc.subject EFFICIENCY en
dc.subject REPAIR en
dc.subject GENES en
dc.subject 3111 Biomedicine en
dc.title CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response en
dc.type Article
dc.description.version Peer reviewed
dc.type.uri info:eu-repo/semantics/other
dc.type.uri info:eu-repo/semantics/publishedVersion

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