CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response

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dc.contributor University of Helsinki, Genome-Scale Biology (GSB) Research Program en
dc.contributor University of Helsinki, Doctoral Programme in Integrative Life Science en
dc.contributor.author Haapaniemi, Emma
dc.contributor.author Botla, Sandeep
dc.contributor.author Persson, Jenna
dc.contributor.author Schmierer, Bernhard
dc.contributor.author Taipale, Jussi
dc.date.accessioned 2019-06-30T21:46:16Z
dc.date.available 2021-05-08T21:46:18Z
dc.date.issued 2018-07
dc.identifier.citation Haapaniemi , E , Botla , S , Persson , J , Schmierer , B & Taipale , J 2018 , ' CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response ' , Nature Medicine , vol. 24 , no. 7 , pp. 927-+ . https://doi.org/10.1038/s41591-018-0049-z en
dc.identifier.issn 1078-8956
dc.identifier.other PURE: 114801223
dc.identifier.other PURE UUID: 8f67cc84-e64b-4e1c-84f3-f8fca5cc0ae0
dc.identifier.other WOS: 000438187700019
dc.identifier.other Scopus: 85048349385
dc.identifier.uri http://hdl.handle.net/10138/303675
dc.description.abstract Here, we report that genome editing by CRISPR-Cas9 induces a p53-mediated DNA damage response and cell cycle arrest in immortalized human retinal pigment epithelial cells, leading to a selection against cells with a functional p53 pathway. Inhibition of p53 prevents the damage response and increases the rate of homologous recombination from a donor template. These results suggest that p53 inhibition may improve the efficiency of genome editing of untransformed cells and that p53 function should be monitored when developing cell-based therapies utilizing CRISPR-Cas9. en
dc.format.extent 6
dc.language.iso eng
dc.relation.ispartof Nature Medicine
dc.rights en
dc.subject CELLS en
dc.subject INHIBITION en
dc.subject EFFICIENCY en
dc.subject REPAIR en
dc.subject GENES en
dc.subject 3111 Biomedicine en
dc.title CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response en
dc.type Article
dc.description.version Peer reviewed
dc.identifier.doi https://doi.org/10.1038/s41591-018-0049-z
dc.type.uri info:eu-repo/semantics/other
dc.type.uri info:eu-repo/semantics/publishedVersion
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