Improving Illumina assemblies with Hi-C and long reads : An example with the North African dromedary

Show full item record



Permalink

http://hdl.handle.net/10138/303677

Citation

Elbers , J P , Rogers , M F , Perelman , P L , Proskuryakova , A A , Serdyukova , N A , Johnson , W E , Horin , P , Corander , J , Murphy , D & Burger , P A 2019 , ' Improving Illumina assemblies with Hi-C and long reads : An example with the North African dromedary ' , Molecular Ecology Resources , vol. 19 , no. 4 , pp. 1015-1026 . https://doi.org/10.1111/1755-0998.13020

Title: Improving Illumina assemblies with Hi-C and long reads : An example with the North African dromedary
Author: Elbers, Jean P.; Rogers, Mark F.; Perelman, Polina L.; Proskuryakova, Anastasia A.; Serdyukova, Natalia A.; Johnson, Warren E.; Horin, Petr; Corander, Jukka; Murphy, David; Burger, Pamela A.
Contributor: University of Helsinki, Jukka Corander / Principal Investigator
Date: 2019-07
Language: eng
Number of pages: 12
Belongs to series: Molecular Ecology Resources
ISSN: 1755-098X
URI: http://hdl.handle.net/10138/303677
Abstract: Researchers have assembled thousands of eukaryotic genomes using Illumina reads, but traditional mate-pair libraries cannot span all repetitive elements, resulting in highly fragmented assemblies. However, both chromosome conformation capture techniques, such as Hi-C and Dovetail Genomics Chicago libraries and long-read sequencing, such as Pacific Biosciences and Oxford Nanopore, help span and resolve repetitive regions and therefore improve genome assemblies. One important livestock species of arid regions that does not have a high-quality contiguous reference genome is the dromedary (Camelus dromedarius). Draft genomes exist but are highly fragmented, and a high-quality reference genome is needed to understand adaptation to desert environments and artificial selection during domestication. Dromedaries are among the last livestock species to have been domesticated, and together with wild and domestic Bactrian camels, they are the only representatives of the Camelini tribe, which highlights their evolutionary significance. Here we describe our efforts to improve the North African dromedary genome. We used Chicago and Hi-C sequencing libraries from Dovetail Genomics to resolve the order of previously assembled contigs, producing almost chromosome-level scaffolds. Remaining gaps were filled with Pacific Biosciences long reads, and then scaffolds were comparatively mapped to chromosomes. Long reads added 99.32 Mbp to the total length of the new assembly. Dovetail Chicago and Hi-C libraries increased the longest scaffold over 12-fold, from 9.71 Mbp to 124.99 Mbp and the scaffold N50 over 50-fold, from 1.48 Mbp to 75.02 Mbp. We demonstrate that Illumina de novo assemblies can be substantially upgraded by combining chromosome conformation capture and long-read sequencing.
Subject: 1181 Ecology, evolutionary biology
1184 Genetics, developmental biology, physiology
chromosome mapping
chromosome conformation capture
dromedary
genome assembly
scaffolding
genome annotation
PLANT
CATTLE
ABYSS
PRODUCTION TRAITS
ANNOTATION
SEQUENCE
ARCHITECTURE
GENOME-WIDE ASSOCIATION
RESOURCE
MAKER
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
Elbers_et_al_2019_Molecular_Ecology_Resources.pdf 1.652Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record