Extracellular vesicles from oral squamous carcinoma cells display pro- and anti-angiogenic properties

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http://hdl.handle.net/10138/303875

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de Andrade , A L D L , de Oliveira , C E , Dourado , M R , Macedo , C C S , Winck , F V , Paes Leme , A F , Salo , T , Coletta , R D , de Almeida Freitas , R & Galvao , H C 2018 , ' Extracellular vesicles from oral squamous carcinoma cells display pro- and anti-angiogenic properties ' , Oral Diseases , vol. 24 , no. 5 , pp. 725-731 . https://doi.org/10.1111/odi.12765

Title: Extracellular vesicles from oral squamous carcinoma cells display pro- and anti-angiogenic properties
Author: de Andrade, A. L. D. L.; de Oliveira, C. E.; Dourado, M. R.; Macedo, C. C. S.; Winck, F. V.; Paes Leme, A. F.; Salo, T.; Coletta, R. D.; de Almeida Freitas, R.; Galvao, H. C.
Other contributor: University of Helsinki, Clinicum



Date: 2018-07
Language: eng
Number of pages: 7
Belongs to series: Oral Diseases
ISSN: 1354-523X
DOI: https://doi.org/10.1111/odi.12765
URI: http://hdl.handle.net/10138/303875
Abstract: BackgroundA new intercellular communication mode established by neoplastic cells and tumor microenvironment components is based on extracellular vesicles (EVs). However, the biological effects of the EVs released by tumor cells on angiogenesis are not completely understood. Here, we aimed to understand the biological effects of EVs isolated from two cell lines of oral squamous cell carcinoma (OSCC) (SCC15 and HSC3) on endothelial cell tubulogenesis. MethodsOSCC-derived EVs were isolated with a polymer-based precipitation method, quantified using nanoparticle tracking analysis and verified for EV markers by dot blot. Functional assays were performed to assess the angiogenic potential of the OSCC-derived EVs. ResultsThe results showed that EVs derived from both cell lines displayed typical spherical-shaped morphology and expressed the EV markers CD63 and Annexin II. Although the average particle concentration and size were quite similar, SCC15-derived EVs promoted a pronounced tubular formation associated with significant migration and apoptosis rates of the endothelial cells, whereas EVs derived from HSC3 cells inhibited significantly endothelial cell tubulogenesis and proliferation. ConclusionThe findings of this study reveal that EVs derived from different OSCC cell lines by a polymer-based precipitation method promote pro- or anti-angiogenic effects.
Subject: angiogenesis
extracellular vesicles
oral cancer
TUMOR-DERIVED EXOSOMES
ENDOTHELIAL-CELLS
CANCER
MICROVESICLES
ACTIVATION
HALLMARKS
DISEASE
313 Dentistry
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