Near-Infrared Fluorescent Proteins and Their Applications

Show full item record



Permalink

http://hdl.handle.net/10138/303883

Citation

Karasev , M M , Stepanenko , O V , Rumyantsev , K A , Turoverov , K K & Verkhusha , V V 2019 , ' Near-Infrared Fluorescent Proteins and Their Applications ' , Biochemistry (Moscow) , vol. 84 , pp. 32-50 . https://doi.org/10.1134/S0006297919140037

Title: Near-Infrared Fluorescent Proteins and Their Applications
Author: Karasev, M. M.; Stepanenko, O. V.; Rumyantsev, K. A.; Turoverov, K. K.; Verkhusha, V. V.
Contributor: University of Helsinki, Department of Anatomy
University of Helsinki, Department of Anatomy
Date: 2019-01
Language: eng
Number of pages: 19
Belongs to series: Biochemistry (Moscow)
ISSN: 0006-2979
URI: http://hdl.handle.net/10138/303883
Abstract: High transparency, low light-scattering, and low autofluorescence of mammalian tissues in the near-infrared (NIR) spectral range (650-900 nm) open a possibility for in vivo imaging of biological processes at the micro-and macroscales to address basic and applied problems in biology and biomedicine. Recently, probes that absorb and fluoresce in the NIR optical range have been engineered using bacterial phytochromes-natural NIR light-absorbing photoreceptors that regulate metabolism in bacteria. Since the chromophore in all these proteins is biliverdin, a natural product of heme catabolism in mammalian cells, they can be used as genetically encoded fluorescent probes, similarly to GFP-like fluorescent proteins. In this review, we discuss photophysical and biochemical properties of NIR fluorescent proteins, reporters, and biosensors and analyze their characteristics required for expression of these molecules in mammalian cells. Structural features and molecular engineering of NIR fluorescent probes are discussed. Applications of NIR fluorescent proteins and biosensors for studies of molecular processes in cells, as well as for tissue and organ visualization in whole-body imaging in vivo, are described. We specifically focus on the use of NIR fluorescent probes in advanced imaging technologies that combine fluorescence and bioluminescence methods with photoacoustic tomography.
Subject: fluorescent proteins
biomarkers
bacterial phytochromes
in vivo visualization
fluorescence
bioluminescence
biosensors
NF-KAPPA-B
SPATIOTEMPORAL DYNAMICS
BACTERIAL PHYTOCHROMES
FRET BIOSENSORS
PHOTOACOUSTIC TOMOGRAPHY
COMPLEMENTATION BIFC
CHROMOPHORE-BINDING
REPORTER
BACTERIOPHYTOCHROME
CELLS
1183 Plant biology, microbiology, virology
3111 Biomedicine
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
Karasev2019_Art ... raredFluorescentProtei.pdf 2.788Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record