Microfluidic Lateral Flow Cytochrome P450 Assay on a Novel Printed Functionalized Calcium Carbonate-Based Platform for Rapid Screening of Human Xenobiotic Metabolism

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http://hdl.handle.net/10138/303908

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Jutila , E , Koivunen , R , Kiiski , I , Bollström , R , Sikanen , T & Gane , P 2018 , ' Microfluidic Lateral Flow Cytochrome P450 Assay on a Novel Printed Functionalized Calcium Carbonate-Based Platform for Rapid Screening of Human Xenobiotic Metabolism ' , Advanced Functional Materials , vol. 28 , no. 31 , 1802793 . https://doi.org/10.1002/adfm.201802793

Title: Microfluidic Lateral Flow Cytochrome P450 Assay on a Novel Printed Functionalized Calcium Carbonate-Based Platform for Rapid Screening of Human Xenobiotic Metabolism
Author: Jutila, Eveliina; Koivunen, Risto; Kiiski, Iiro; Bollström, Roger; Sikanen, Tiina; Gane, Patrick
Contributor: University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
Date: 2018-08-01
Language: eng
Number of pages: 11
Belongs to series: Advanced Functional Materials
ISSN: 1616-301X
URI: http://hdl.handle.net/10138/303908
Abstract: Cytochrome P450 (CYP) is a superfamily of enzymes in charge of elimination of the majority of clinically used drugs and other xenobiotics. This study focuses on the development of a rapid microfluidic lateral flow assay to study human phase I metabolism reactions mediated by CYP2A6 isoenzyme, the major detoxification route for many known carcinogens and drugs, with coumarin 7-hydroxylation, as the prototype model reaction. Assay fabrication utilizes custom-designed porous functionalized calcium carbonate (FCC) coatings and inkjet-printed fluid barriers. All materials used are novel and carefully chosen to preserve biocompatibility. The design comprises separate zones for reaction, separation and detection, and an absorbent pad to keep the assay wet for extended periods (up to 10 min) even when heated to physiological temperature. The concept enables CYP assays to be made at lower cost than conventional well-plate assays, while providing increased selectivity at equally high speed, owing to the possibility for simultaneous chromatographic separation of the reaction products from the reactants on the FCC coating. The developed concept provides a viable rapid prediction of the interaction risks related to metabolic clearance of drugs and other xenobiotics, and exemplifies a novel coating technology illustrating the opportunity to broaden application functionality.
Subject: cytochrome P450
drug metabolism
functional coatings
inkjet printing
porous materials
PAPER-BASED MICROFLUIDICS
LIQUID UPTAKE CAPACITY
ANALYTICAL DEVICES
FABRICATION
ENZYME
HYDROXYAPATITE
COATINGS
GLUCOSE
CARE
WAX
116 Chemical sciences
221 Nano-technology
317 Pharmacy
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