Interleukin-17F Has Anti-Tumor Effects in Oral Tongue Cancer

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http://hdl.handle.net/10138/303948

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Almahmoudi , R , Salem , A , Murshid , S , Dourado , M R , Apu , E H , Salo , T & Al-Samadi , A 2019 , ' Interleukin-17F Has Anti-Tumor Effects in Oral Tongue Cancer ' , Cancers , vol. 11 , no. 5 , 650 . https://doi.org/10.3390/cancers11050650

Title: Interleukin-17F Has Anti-Tumor Effects in Oral Tongue Cancer
Author: Almahmoudi, Rabeia; Salem, Abdelhakim; Murshid, Sakhr; Dourado, Mauricio Rocha; Apu, Ehsanul Hoque; Salo, Tuula; Al-Samadi, Ahmed
Contributor: University of Helsinki, Department of Oral and Maxillofacial Diseases
University of Helsinki, HUS Internal Medicine and Rehabilitation
University of Helsinki, Clinicum
University of Helsinki, Department of Oral and Maxillofacial Diseases
University of Helsinki, Department of Oral and Maxillofacial Diseases
Date: 2019-05
Language: eng
Number of pages: 13
Belongs to series: Cancers
ISSN: 2072-6694
URI: http://hdl.handle.net/10138/303948
Abstract: We recently showed that extracellular interleukin-17F (IL-17F) correlates with better disease-specific survival in oral tongue squamous cell carcinoma (OTSCC) patients. However, the underlying mechanisms of such effect remain obscure. Here, we used qRT-PCR to assess the expression of IL-17F and its receptors (IL-17RA and IL-17RC) in two OTSCC cell lines (HSC-3 and SCC-25) and in normal human oral keratinocytes (HOKs). IL-17F effects on cancer cell proliferation, migration, and invasion were studied using a live-imaging IncuCyte system, and a Caspase-3/7 reagent was used for testing apoptosis. 3D tumor spheroids were utilized to assess the impact of IL-17F on invasion with or without cancer-associated fibroblasts (CAFs). Tube-formation assays were used to examine the effects of IL-17F on angiogenesis using human umbilical vein endothelial cells (HUVEC). OTSCC cells express low levels of IL-17F, IL-17RA, and IL-17RC mRNA compared with HOKs. IL-17F inhibited cell proliferation and random migration of highly invasive HSC-3 cells. CAFs promoted OTSCC invasion in tumor spheroids, whereas IL-17F eliminated such effect. IL-17F suppressed HUVEC tube formation in a dose-dependent manner. Collectively, we suggest that IL-17F counteracts the pro-tumorigenic activity in OTSCC. Due to its downregulation in tumor cells and inhibitory activity in in vitro cancer models, targeting IL-17F or its regulatory pathways could lead to promising immunotherapeutic strategies against OTSCC.
Subject: interleukin-17F
cancer immunotherapy
cytokine therapy
tongue cancer
oral squamous cell carcinoma
proliferation
invasion
apoptosis
angiogenesis
HUVEC cells
CELL-CYCLE
T-CELLS
IL-17
CARCINOMA
MIGRATION
PROMOTES
TRENDS
GROWTH
CAVITY
TH17
3122 Cancers
3125 Otorhinolaryngology, ophthalmology
3111 Biomedicine
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