Almahmoudi , R , Salem , A , Murshid , S , Dourado , M R , Apu , E H , Salo , T & Al-Samadi , A 2019 , ' Interleukin-17F Has Anti-Tumor Effects in Oral Tongue Cancer ' , Cancers , vol. 11 , no. 5 , 650 . https://doi.org/10.3390/cancers11050650
Title: | Interleukin-17F Has Anti-Tumor Effects in Oral Tongue Cancer |
Author: | Almahmoudi, Rabeia; Salem, Abdelhakim; Murshid, Sakhr; Dourado, Mauricio Rocha; Apu, Ehsanul Hoque; Salo, Tuula; Al-Samadi, Ahmed |
Contributor organization: | Department of Oral and Maxillofacial Diseases TRIMM - Translational Immunology Research Program Research Programs Unit HUS Internal Medicine and Rehabilitation Clinicum |
Date: | 2019-05 |
Language: | eng |
Number of pages: | 13 |
Belongs to series: | Cancers |
ISSN: | 2072-6694 |
DOI: | https://doi.org/10.3390/cancers11050650 |
URI: | http://hdl.handle.net/10138/303948 |
Abstract: | We recently showed that extracellular interleukin-17F (IL-17F) correlates with better disease-specific survival in oral tongue squamous cell carcinoma (OTSCC) patients. However, the underlying mechanisms of such effect remain obscure. Here, we used qRT-PCR to assess the expression of IL-17F and its receptors (IL-17RA and IL-17RC) in two OTSCC cell lines (HSC-3 and SCC-25) and in normal human oral keratinocytes (HOKs). IL-17F effects on cancer cell proliferation, migration, and invasion were studied using a live-imaging IncuCyte system, and a Caspase-3/7 reagent was used for testing apoptosis. 3D tumor spheroids were utilized to assess the impact of IL-17F on invasion with or without cancer-associated fibroblasts (CAFs). Tube-formation assays were used to examine the effects of IL-17F on angiogenesis using human umbilical vein endothelial cells (HUVEC). OTSCC cells express low levels of IL-17F, IL-17RA, and IL-17RC mRNA compared with HOKs. IL-17F inhibited cell proliferation and random migration of highly invasive HSC-3 cells. CAFs promoted OTSCC invasion in tumor spheroids, whereas IL-17F eliminated such effect. IL-17F suppressed HUVEC tube formation in a dose-dependent manner. Collectively, we suggest that IL-17F counteracts the pro-tumorigenic activity in OTSCC. Due to its downregulation in tumor cells and inhibitory activity in in vitro cancer models, targeting IL-17F or its regulatory pathways could lead to promising immunotherapeutic strategies against OTSCC. |
Subject: |
interleukin-17F
cancer immunotherapy cytokine therapy tongue cancer oral squamous cell carcinoma proliferation invasion apoptosis angiogenesis HUVEC cells CELL-CYCLE T-CELLS IL-17 CARCINOMA MIGRATION PROMOTES TRENDS GROWTH CAVITY TH17 3122 Cancers 3125 Otorhinolaryngology, ophthalmology 3111 Biomedicine |
Peer reviewed: | Yes |
Rights: | cc_by |
Usage restriction: | openAccess |
Self-archived version: | publishedVersion |
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