Acetylated nanocellulose for single-component bioinks and cell proliferation on 3D-printed scaffolds

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dc.contributor University of Helsinki, Divisions of Faculty of Pharmacy en
dc.contributor University of Helsinki, Division of Pharmaceutical Chemistry and Technology en
dc.contributor.author Ajdary, Rubina
dc.contributor.author Huan, Siqi
dc.contributor.author Zanjanizadeh Ezazi, Nazanin
dc.contributor.author Xiang, Wenchao
dc.contributor.author Grande, Rafael
dc.contributor.author Santos, Hélder A.
dc.contributor.author Rojas, Orlando J.
dc.date.accessioned 2019-08-02T13:50:02Z
dc.date.available 2019-08-02T13:50:02Z
dc.date.issued 2019-07
dc.identifier.citation Ajdary , R , Huan , S , Zanjanizadeh Ezazi , N , Xiang , W , Grande , R , Santos , H A & Rojas , O J 2019 , ' Acetylated nanocellulose for single-component bioinks and cell proliferation on 3D-printed scaffolds ' , Biomacromolecules , vol. 20 , no. 7 , pp. 2770-2778 . https://doi.org/10.1021/acs.biomac.9b00527 en
dc.identifier.issn 1525-7797
dc.identifier.other PURE: 124809737
dc.identifier.other PURE UUID: c6a7b14f-b6fa-4184-a9fa-8f8e625d23c1
dc.identifier.other WOS: 000474812200031
dc.identifier.uri http://hdl.handle.net/10138/304247
dc.description.abstract Nanocellulose has been demonstrated as a suitable material for cell culturing, given its similarity to extracellular matrices. Taking advantage of the shear thinning behavior, nanocellulose suits three-dimensional (3D) printing into scaffolds that support cell attachment and proliferation. Here, we propose aqueous suspensions of acetylated nanocellulose of a low degree of substitution for direct ink writing (DM). This benefits from the heterogeneous acetylation of precursor cellulosic fibers, which eases their deconstruction and confers the characteristics required for extrusion in DIW. Accordingly, the morphology of related 3D printed architectures and their performance during drying and rewetting as well as interactions with living cells are compared with those produced from typical unmodified and TEMPO-oxidized nanocelluloses. We find that a significantly lower concentration of acetylated nanofibrils is needed to obtain bioinks of similar performance, affording more porous structures. Together with their high surface charge and axial aspect, acetylated nanocellulose produces dimensionally stable monolithic scaffolds that support drying and rewetting, required for packaging and sterilization. Considering their potential uses in cardiac devices, we discuss the interactions of the scaffolds with cardiac myoblast cells. Attachment, proliferation, and viability for 21 days are demonstrated. Overall, the performance of acetylated nanocellulose bioinks opens the possibility for reliable and scaleup fabrication of scaffolds appropriate for studies on cellular processes and for tissue engineering. en
dc.format.extent 9
dc.language.iso eng
dc.relation.ispartof Biomacromolecules
dc.rights en
dc.subject CELLULOSE NANOFIBRILS en
dc.subject DIFFERENTIATION en
dc.subject HYDROGEL en
dc.subject NANOCRYSTALS en
dc.subject STRENGTH en
dc.subject 1182 Biochemistry, cell and molecular biology en
dc.subject 116 Chemical sciences en
dc.subject 216 Materials engineering en
dc.title Acetylated nanocellulose for single-component bioinks and cell proliferation on 3D-printed scaffolds en
dc.type Article
dc.description.version Peer reviewed
dc.identifier.doi https://doi.org/10.1021/acs.biomac.9b00527
dc.type.uri info:eu-repo/semantics/other
dc.type.uri info:eu-repo/semantics/publishedVersion
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