Data on Quantitative Microbial Elemental Cycling (QMEC) primer design and validation

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http://hdl.handle.net/10138/304413

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Zheng , B-X & Ding , K 2019 , ' Data on Quantitative Microbial Elemental Cycling (QMEC) primer design and validation ' , Data in Brief , vol. 23 , 103820 . https://doi.org/10.1016/j.dib.2019.103820

Title: Data on Quantitative Microbial Elemental Cycling (QMEC) primer design and validation
Author: Zheng, Bang-Xiao; Ding, Kai
Contributor: University of Helsinki, Ecosystems and Environment Research Programme
Date: 2019-04
Language: eng
Number of pages: 4
Belongs to series: Data in Brief
ISSN: 2352-3409
URI: http://hdl.handle.net/10138/304413
Abstract: This work included the primer design details of Quantitative Microbial Elemental Cycling (QMEC) method, a high-throughput qPCR method for microbial carbon (C), nitrogen (N), phosphorus (P), sulfur (S) and methane metabolism potential detection and assessment. We designed 36 novel primers based on their amino acid sequences. Via illumina sequencing technology, their phylogenetic taxonomy was identified and analyzed to validate the primer specificity.
Subject: 1183 Plant biology, microbiology, virology
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