Interaction of methyl viologen-induced chloroplast and mitochondrial signalling in Arabidopsis

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Cui , F , Brosche , M , Shapiguzov , A , He , X-Q , Vainonen , J P , Leppala , J , Trotta , A , Kangasjarvi , S , Salojarvi , J , Kangasjarvi , J & Overmyer , K 2019 , ' Interaction of methyl viologen-induced chloroplast and mitochondrial signalling in Arabidopsis ' , Free Radical Biology & Medicine , vol. 134 , pp. 555-566 . https://doi.org/10.1016/j.freeradbiomed.2019.02.006

Title: Interaction of methyl viologen-induced chloroplast and mitochondrial signalling in Arabidopsis
Author: Cui, Fuqiang; Brosche, Mikael; Shapiguzov, Alexey; He, Xin-Qiang; Vainonen, Julia P.; Leppala, Johanna; Trotta, Andrea; Kangasjarvi, Saijaliisa; Salojarvi, Jarkko; Kangasjarvi, Jaakko; Overmyer, Kirk
Contributor: University of Helsinki, Plant Biology
University of Helsinki, Organismal and Evolutionary Biology Research Programme
University of Helsinki, Plant ROS-Signalling
University of Helsinki, Organismal and Evolutionary Biology Research Programme
University of Helsinki, Plant Biology
University of Helsinki, University of Turku
University of Helsinki, Viikki Plant Science Centre (ViPS)
University of Helsinki, Plant Biology
University of Helsinki, Viikki Plant Science Centre (ViPS)
Date: 2019-04
Language: eng
Number of pages: 12
Belongs to series: Free Radical Biology & Medicine
ISSN: 0891-5849
URI: http://hdl.handle.net/10138/304552
Abstract: Reactive oxygen species (ROS) are key signalling intermediates in plant metabolism, defence, and stress adaptation. In plants, both the chloroplast and mitochondria are centres of metabolic control and ROS production, which coordinate stress responses in other cell compartments. The herbicide and experimental tool, methyl viologen (MV) induces ROS generation in the chloroplast under illumination, but is also toxic in non-photosynthetic organisms. We used MV to probe plant ROS signalling in compartments other than the chloroplast. Taking a genetic approach in the model plant Arabidopsis (Arabidopsis thaliana), we used natural variation, QTL mapping, and mutant studies with MV in the light, but also under dark conditions, when the chloroplast electron transport is inactive. These studies revealed a light-independent MV-induced ROS-signalling pathway, suggesting mitochondrial involvement. Mitochondrial Mn SUPEROXIDE DISMUTASE was required for ROS-tolerance and the effect of MV was enhanced by exogenous sugar, providing further evidence for the role of mitochondria. Mutant and hormone feeding assays revealed roles for stress hormones in organellar ROS-responses. The radical-induced cell death1 mutant, which is tolerant to MV-induced ROS and exhibits altered mitochondrial signalling, was used to probe interactions between organelles. Our studies suggest that mitochondria are involved in the response to ROS induced by MV in plants.
Subject: Arabidopsis thaliana
Chloroplast
Natural variation
Methyl viologen
Mitochondria
Paraquat
RADICAL-INDUCED CELL DEATH1
Retrograde signalling
ROS
MANGANESE SUPEROXIDE-DISMUTASE
OXIDATIVE STRESS TOLERANCE
NAC TRANSCRIPTION FACTOR
PARAQUAT TOLERANCE
PHOTOOXIDATIVE STRESS
ELECTRON-TRANSPORT
ABIOTIC STRESS
RESISTANCE
ACID
METABOLISM
1182 Biochemistry, cell and molecular biology
1183 Plant biology, microbiology, virology
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