First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid-chromatography-Urgent need for harmonisation of analytical methods

Show full item record



Permalink

http://hdl.handle.net/10138/304740

Citation

Luetjohann , D , Björkhem , I , Friedrichs , S , Kerksiek , A , Lovgren-Sandblom , A , Geilenkeuser , W-J , Ahrends , R , Andrade , I , Ansorena , D , Astiasaran , I , Baila-Rueda , L , Barriuso , B , Becker , S , Bretillon , L , Browne , R W , Caccia , C , Ceglarek , U , Cenarro , A , Crick , P J , Fauler , G , Garcia-Llatas , G , Gray , R , Griffiths , W J , Gylling , H , Harding , S , Helmschrodt , C , Iuliano , L , Janssen , H-G , Jones , P , Kaipiainen , L , Kannenberg , F , Jesus Lagarda , M , Leoni , V , Lottenberg , A M , MacKay , D S , Matysik , S , McDonald , J , Menendez-Carreno , M , Myrie , S B , Nunes , V S , Ostlund , R E , Polisecki , E , Ramos , F , Rideout , T C , Schaefer , E J , Schmitz , G , Wang , Y , Zerbinati , C , Diczfalusy , U & Schött , H-F 2019 , ' First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid-chromatography-Urgent need for harmonisation of analytical methods ' , Journal of Steroid Biochemistry and Molecular Biology , vol. 190 , pp. 115-125 . https://doi.org/10.1016/j.jsbmb.2019.03.025

Title: First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid-chromatography-Urgent need for harmonisation of analytical methods
Author: Luetjohann, Dieter; Björkhem, Ingemar; Friedrichs, Silvia; Kerksiek, Anja; Lovgren-Sandblom, Anita; Geilenkeuser, Wolf-Jochen; Ahrends, Robert; Andrade, Isabel; Ansorena, Diana; Astiasaran, Iciar; Baila-Rueda, Lucia; Barriuso, Bianca; Becker, Susen; Bretillon, Lionel; Browne, Richard W.; Caccia, Claudio; Ceglarek, Uta; Cenarro, Ana; Crick, Peter J.; Fauler, Günter; Garcia-Llatas, Guadalupe; Gray, Robert; Griffiths, William J.; Gylling, Helena; Harding, Scott; Helmschrodt, Christin; Iuliano, Luigi; Janssen, Hans-Gerd; Jones, Peter; Kaipiainen, Leena; Kannenberg, Frank; Jesus Lagarda, Maria; Leoni, Valerio; Lottenberg, Ana Maria; MacKay, Dylan S.; Matysik, Silke; McDonald, Jeff; Menendez-Carreno, Maria; Myrie, Semone B.; Nunes, Valeria Sutti; Ostlund, Richard E.; Polisecki, Eliana; Ramos, Fernando; Rideout, Todd C.; Schaefer, Ernst J.; Schmitz, Gerd; Wang, Yuqin; Zerbinati, Chiara; Diczfalusy, Ulf; Schött, Hans-Frieder
Contributor: University of Helsinki, University of Helsinki
University of Helsinki, Department of Medicine
Date: 2019-06
Language: eng
Number of pages: 11
Belongs to series: Journal of Steroid Biochemistry and Molecular Biology
ISSN: 0960-0760
URI: http://hdl.handle.net/10138/304740
Abstract: Serum concentrations of lathosterol, the plant sterols campesterol and sitosterol and the cholesterol metabolite 5 alpha-cholestanol are widely used as surrogate markers of cholesterol synthesis and absorption, respectively. Increasing numbers of laboratories utilize a broad spectrum of well-established and recently developed methods for the determination of cholesterol and non-cholesterol sterols (NCS). In order to evaluate the quality of these measurements and to identify possible sources of analytical errors our group initiated the first international survey for cholesterol and NCS. The cholesterol and NCS survey was structured as a two-part survey which took place in the years 2013 and 2014. The first survey part was designed as descriptive, providing information about the variation of reported results from different laboratories. A set of two lyophilized pooled sera (A and B) was sent to twenty laboratories specialized in chromatographic lipid analysis. The different sterols were quantified either by gas chromatography-flame ionization detection, gas chromatography- or liquid chromatography-mass selective detection. The participants were requested to determine cholesterol and NCS concentrations in the provided samples as part of their normal laboratory routine. The second part was designed as interventional survey. Twenty-two laboratories agreed to participate and received again two different lyophilized pooled sera (C and D). In contrast to the first international survey, each participant received standard stock solutions with defined concentrations of cholesterol and NCS. The participants were requested to use diluted calibration solutions from the provided standard stock solutions for quantification of cholesterol and NCS. In both surveys, each laboratory used its own internal standard (5 alpha-cholestane, epicoprostanol or deuterium labelled sterols). Main outcome of the survey was, that unacceptably high interlaboratory variations for cholesterol and NCS concentrations are reported, even when the individual laboratories used the same calibration material. We discuss different sources of errors and recommend all laboratories analysing cholesterol and NCS to participate in regular quality control programs.
Subject: Surrogate marker
Cholesterol absorption
Cholesterol synthesis
Cholesterol balance
Atherosclerosis
Phytosterols
SERUM PLANT STEROLS
ABSORPTION
PRECURSORS
3121 General medicine, internal medicine and other clinical medicine
1182 Biochemistry, cell and molecular biology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
1_s2.0_S0960076019301293_main.pdf 2.647Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record