Evaluation of Real-Time RT-PCR for Diagnostic Use in Detection of Puumala Virus

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Niskanen , S , Jääskeläinen , A , Vapalahti , O & Sironen , T 2019 , ' Evaluation of Real-Time RT-PCR for Diagnostic Use in Detection of Puumala Virus ' , Viruses (Basel) , vol. 11 , no. 7 , 661 . https://doi.org/10.3390/v11070661

Title: Evaluation of Real-Time RT-PCR for Diagnostic Use in Detection of Puumala Virus
Author: Niskanen, Silja; Jääskeläinen, Anne; Vapalahti, Olli; Sironen, Tarja
Contributor organization: Department of Virology
University of Helsinki
Staff Services
Viral Zoonosis Research Unit
Veterinary Biosciences
Helsinki One Health (HOH)
Olli Pekka Vapalahti / Principal Investigator
Veterinary Microbiology and Epidemiology
Emerging Infections Research Group
Date: 2019-07
Language: eng
Number of pages: 9
Belongs to series: Viruses (Basel)
ISSN: 1999-4915
DOI: https://doi.org/10.3390/v11070661
URI: http://hdl.handle.net/10138/304840
Abstract: Puumala virus (PUUV) is the most common cause of hantavirus infection in Europe, with thousands of cases occurring particularly in Northern, Central and Eastern Europe and Russia. It causes a mild form of hemorrhagic fever with renal syndrome also known as nephropathia epidemica (NE) with clinical picture ranging from mild to severe. Currently, the laboratory diagnosis of NE is mainly based on serology. Here, we evaluated a real-time one-step qRT-PCR (PUUV-qRT-PCR) for detection of PUUV with 238 consecutive diagnostic serum samples from patients with suspected PUUV infection. The PUUV-qRT-PCR was both specific and sensitive for PUUV RNA. The analytical sensitivity (limit of detection) was estimated to be four copies of PUUV per reaction. Altogether 28 out of 30 (93%) PUUV IgM positive samples were positive also for PUUV RNA. No false positives were detected and the specificity was thus 100%. Interestingly, one sample was found positive in PUUV-qRT-PCR prior to subsequent IgM and IgG seroconversion. PUUV-qRT-PCR could be used for diagnostics in the early phase of NE infection and might be helpful especially in the rare severe cases when the patient's condition may deteriorate rapidly.
Subject: Puumala virus
real-time qRT-PCR
nephropathia epidemica
1183 Plant biology, microbiology, virology
3142 Public health care science, environmental and occupational health
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

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