Surface PEGylation suppresses pulmonary effects of CuO in allergen-induced lung inflammation

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Ilves , M , Kinaret , P A S , Ndika , J , Karisola , P , Marwah , V , Fortino , V , Fedutik , Y , Correia , M , Ehrlich , N , Loeschner , K , Besinis , A , Vassallo , J , Handy , R D , Wolff , H , Savolainen , K , Greco , D & Alenius , H 2019 , ' Surface PEGylation suppresses pulmonary effects of CuO in allergen-induced lung inflammation ' , Particle and Fibre Toxicology , vol. 16 , 28 . https://doi.org/10.1186/s12989-019-0309-1

Title: Surface PEGylation suppresses pulmonary effects of CuO in allergen-induced lung inflammation
Author: Ilves, Marit; Kinaret, Pia Anneli Sofia; Ndika, Joseph; Karisola, Piia; Marwah, Veer; Fortino, Vittorio; Fedutik, Yuri; Correia, Manuel; Ehrlich, Nicky; Loeschner, Katrin; Besinis, Alexandros; Vassallo, Joanne; Handy, Richard D.; Wolff, Henrik; Savolainen, Kai; Greco, Dario; Alenius, Harri
Contributor: University of Helsinki, HUMI - Human Microbiome Research
University of Helsinki, Institute of Biotechnology
University of Helsinki, HUMI - Human Microbiome Research
University of Helsinki, HUMI - Human Microbiome Research
University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
University of Helsinki, Medicum
University of Helsinki, Institute of Biotechnology
University of Helsinki, HUMI - Human Microbiome Research
Date: 2019-07-05
Language: eng
Number of pages: 21
Belongs to series: Particle and Fibre Toxicology
ISSN: 1743-8977
URI: http://hdl.handle.net/10138/305140
Abstract: BackgroundCopper oxide (CuO) nanomaterials are used in a wide range of industrial and commercial applications. These materials can be hazardous, especially if they are inhaled. As a result, the pulmonary effects of CuO nanomaterials have been studied in healthy subjects but limited knowledge exists today about their effects on lungs with allergic airway inflammation (AAI). The objective of this study was to investigate how pristine CuO modulates allergic lung inflammation and whether surface modifications can influence its reactivity.CuO and its carboxylated (CuO COOH), methylaminated (CuO NH3) and PEGylated (CuO PEG) derivatives were administered here on four consecutive days via oropharyngeal aspiration in a mouse model of AAI. Standard genome-wide gene expression profiling as well as conventional histopathological and immunological methods were used to investigate the modulatory effects of the nanomaterials on both healthy and compromised immune system.ResultsOur data demonstrates that although CuO materials did not considerably influence hallmarks of allergic airway inflammation, the materials exacerbated the existing lung inflammation by eliciting dramatic pulmonary neutrophilia. Transcriptomic analysis showed that CuO, CuO COOH and CuO NH3 commonly enriched neutrophil-related biological processes, especially in healthy mice. In sharp contrast, CuO PEG had a significantly lower potential in triggering changes in lungs of healthy and allergic mice revealing that surface PEGylation suppresses the effects triggered by the pristine material.ConclusionsCuO as well as its functionalized forms worsen allergic airway inflammation by causing neutrophilia in the lungs, however, our results also show that surface PEGylation can be a promising approach for inhibiting the effects of pristine CuO. Our study provides information for health and safety assessment of modified CuO materials, and it can be useful in the development of nanomedical applications.
Subject: CuO
Engineered nanomaterial
Health effects
Inflammation
Asthma
Allergic airway inflammation
Risk assessment
COPPER-OXIDE NANOPARTICLES
WALLED CARBON NANOTUBES
EPITHELIAL-CELLS
AIRWAY INFLAMMATION
INHALATION EXPOSURE
GENE-EXPRESSION
TOXICITY
ASTHMA
NANOMATERIALS
PARTICLES
3111 Biomedicine
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