Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish

Show full item record



Permalink

http://hdl.handle.net/10138/305259

Citation

Narumanchi , S , Kalervo , K , Perttunen , S , Wang , H , Immonen , K , Kosonen , R , Laine , M , Ruskoaho , H , Tikkanen , I , Lakkisto , P & Paavola , J 2019 , ' Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish ' , Journal of Cardiovascular Development and Disease , vol. 6 , no. 2 , 16 . https://doi.org/10.3390/jcdd6020016

Title: Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish
Author: Narumanchi, Suneeta; Kalervo, Karri; Perttunen, Sanni; Wang, Hong; Immonen, Katariina; Kosonen, Riikka; Laine, Mika; Ruskoaho, Heikki; Tikkanen, Ilkka; Lakkisto, Päivi; Paavola, Jere
Contributor: University of Helsinki, Medicum
University of Helsinki, Medicum
University of Helsinki, Medicum
University of Helsinki, HUS Heart and Lung Center
University of Helsinki, Regenerative pharmacology group
University of Helsinki, Department of Medicine
University of Helsinki, Neurologian yksikkö
University of Helsinki, HUS Neurocenter
Date: 2019-06
Language: eng
Number of pages: 12
Belongs to series: Journal of Cardiovascular Development and Disease
ISSN: 2308-3425
URI: http://hdl.handle.net/10138/305259
Abstract: The let-7c family of micro-RNAs (miRNAs) is expressed during embryonic development and plays an important role in cell differentiation. We have investigated the role of let-7c in heart regeneration after injury in adult zebrafish. let-7c antagomir or scramble injections were given at one day after cryoinjury (1 dpi). Tissue samples were collected at 7 dpi, 14 dpi and 28 dpi and cardiac function was assessed before cryoinjury, 1 dpi, 7 dpi, 14 dpi and 28 dpi. Inhibition of let-7c increased the rate of fibrinolysis, increased the number of proliferating cell nuclear antigen (PCNA) positive cardiomyocytes at 7 dpi and increased the expression of the epicardial marker raldh2 at 7 dpi. Additionally, cardiac function measured with echocardiography recovered slightly more rapidly after inhibition of let-7c. These results reveal a beneficial role of let-7c inhibition in adult zebrafish heart regeneration.
Subject: let-7c
miRNAs
epicardial cells
cardiac regeneration
proliferating cardiomyocytes
fibrin
HEART REGENERATION
CARDIOVASCULAR-DISEASE
MICRORNA THERAPEUTICS
DIFFERENTIATION
ENDOCARDIUM
EPICARDIUM
CAPACITY
FAMILY
SEPT7B
CELLS
3121 General medicine, internal medicine and other clinical medicine
3111 Biomedicine
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
jcdd_06_00016_published_4.4.19.pdf 4.141Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record