Twins with different personalities : STAT5B-but not STAT5A-has a key role in BCR/ABL-induced leukemia

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Kollmann , S , Grundschober , E , Maurer , B , Warsch , W , Grausenburger , R , Edlinger , L , Huuhtanen , J , Lagger , S , Hennighausen , L , Valent , P , Decker , T , Strobl , B , Mueller , M , Mustjoki , S , Hoelbl-Kovacic , A & Sexl , V 2019 , ' Twins with different personalities : STAT5B-but not STAT5A-has a key role in BCR/ABL-induced leukemia ' , Leukemia , vol. 33 , no. 7 , pp. 1583-1597 .

Title: Twins with different personalities : STAT5B-but not STAT5A-has a key role in BCR/ABL-induced leukemia
Author: Kollmann, Sebastian; Grundschober, Eva; Maurer, Barbara; Warsch, Wolfgang; Grausenburger, Reinhard; Edlinger, Leo; Huuhtanen, Jani; Lagger, Sabine; Hennighausen, Lothar; Valent, Peter; Decker, Thomas; Strobl, Birgit; Mueller, Mathias; Mustjoki, Satu; Hoelbl-Kovacic, Andrea; Sexl, Veronika
Contributor organization: Director and Common Matters
Department of Clinical Chemistry and Hematology
University of Helsinki
Department of Oncology
HUS Comprehensive Cancer Center
University Management
Date: 2019-07
Language: eng
Number of pages: 15
Belongs to series: Leukemia
ISSN: 0887-6924
Abstract: Deregulation of the Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway is found in cancer with STAT5A/B controlling leukemic cell survival and disease progression. As mutations in STAT5B, but not STAT5A, have been frequently described in hematopoietic tumors, we used BCR/ABL as model systems to investigate the contribution of STAT5A or STAT5B for leukemogenesis. The absence of STAT5A decreased cell survival and colony formation. Even more drastic effects were observed in the absence of STAT5B. STAT5B-deficient cells formed BCR/ABL(+) colonies or stable cell lines at low frequency. The rarely evolving Stat5b(-/-) cell lines expressed enhanced levels of BCR/ABL oncoprotein compared to wild-type cells. In line, Stat5b(-/-) leukemic cells induced leukemia with a significantly prolonged disease onset, whereas Stat5a(-/-) cells rapidly caused a fatal disease superimposable to wild-type cells. RNA-sequencing (RNA-seq) profiling revealed a marked enhancement of interferon (IFN)-alpha and IFN-gamma signatures in Stat5b(-/-) cells. Inhibition of IFN responses rescued BCR/ABL(+) colony formation of Stat5b(-/-)-deficient cells. A downregulated IFN response was also observed in patients suffering from leukemia carrying STAT5B mutations. Our data define STAT5B as major STAT5 isoform driving BCR/ABL(+) leukemia. STAT5B enables transformation by suppressing IFN-alpha/gamma, thereby facilitating leukemogenesis. Our findings might help explain the high frequency of STAT5B mutations in hematopoietic tumors.
3122 Cancers
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

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