Lethal Interaction of Nuclear and Mitochondrial Genotypes in Drosophila melanogaster

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dc.contributor.author Salminen, Tiina S.
dc.contributor.author Cannino, Giuseppe
dc.contributor.author Oliveira, Marcos T.
dc.contributor.author Lillsunde, Päivi
dc.contributor.author Jacobs, Howard T.
dc.contributor.author Kaguni, Laurie S.
dc.date.accessioned 2019-09-12T12:41:01Z
dc.date.available 2019-09-12T12:41:01Z
dc.date.issued 2019-07
dc.identifier.citation Salminen , T S , Cannino , G , Oliveira , M T , Lillsunde , P , Jacobs , H T & Kaguni , L S 2019 , ' Lethal Interaction of Nuclear and Mitochondrial Genotypes in Drosophila melanogaster ' , G3 - Genes genomes genetics , vol. 9 , no. 7 , pp. 2225-2234 . https://doi.org/10.1534/g3.119.400315
dc.identifier.other PURE: 126719044
dc.identifier.other PURE UUID: c1c80138-3981-4bd4-bb54-e6eb47fa6b79
dc.identifier.other WOS: 000475396300016
dc.identifier.uri http://hdl.handle.net/10138/305408
dc.description.abstract Drosophila melanogaster, like most animal species, displays considerable genetic variation in both nuclear and mitochondrial DNA (mtDNA). Here we tested whether any of four natural mtDNA variants was able to modify the effect of the phenotypically mild, nuclear tko(25t) mutation, affecting mitochondrial protein synthesis. When combined with tko(25t), the mtDNA from wild strain KSA2 produced pupal lethality, accompanied by the presence of melanotic nodules in L3 larvae. KSA2 mtDNA, which carries a substitution at a conserved residue of cytochrome b that is predicted to be involved in subunit interactions within respiratory complex III, conferred drastically decreased respiratory capacity and complex III activity in the tko(25t) but not a wild-type nuclear background. The complex III inhibitor antimycin A was able to phenocopy effects of the tko(25t) mutation in the KSA2 mtDNA background. This is the first report of a lethal, nuclear-mitochondrial interaction within a metazoan species, representing a paradigm for understanding genetic interactions between nuclear and mitochondrial genotype relevant to human health and disease. en
dc.format.extent 10
dc.language.iso eng
dc.relation.ispartof G3 - Genes genomes genetics
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject cybrid
dc.subject respiration
dc.subject cytochrome b
dc.subject mtDNA copy number
dc.subject melanotic nodules
dc.subject PHENOTYPE
dc.subject MUTATION
dc.subject DNA
dc.subject RESISTANCE
dc.subject DISEASE
dc.subject LOCUS
dc.subject MODEL
dc.subject REPLACEMENT
dc.subject 1184 Genetics, developmental biology, physiology
dc.title Lethal Interaction of Nuclear and Mitochondrial Genotypes in Drosophila melanogaster en
dc.type Article
dc.contributor.organization Doctoral Programme in Integrative Life Science
dc.contributor.organization Institute of Biotechnology
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1534/g3.119.400315
dc.relation.issn 2160-1836
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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