Unique transcriptional and protein-expression signature in human lung tissue-resident NK cells

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http://hdl.handle.net/10138/305442

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Marquardt , N , Kekalainen , E , Chen , P , Lourda , M , Wilson , J N , Scharenberg , M , Bergman , P , Al-Ameri , M , Hard , J , Mold , J E , Ljunggren , H-G & Michaelsson , J 2019 , ' Unique transcriptional and protein-expression signature in human lung tissue-resident NK cells ' , Nature Communications , vol. 10 , 3841 . https://doi.org/10.1038/s41467-019-11632-9

Title: Unique transcriptional and protein-expression signature in human lung tissue-resident NK cells
Author: Marquardt, Nicole; Kekalainen, Eliisa; Chen, Puran; Lourda, Magda; Wilson, Jennifer N.; Scharenberg, Marlena; Bergman, Per; Al-Ameri, Mamdoh; Hard, Joanna; Mold, Jeffrey E.; Ljunggren, Hans-Gustaf; Michaelsson, Jakob
Contributor: University of Helsinki, Department of Bacteriology and Immunology
Date: 2019-08-26
Language: eng
Number of pages: 12
Belongs to series: Nature Communications
ISSN: 2041-1723
URI: http://hdl.handle.net/10138/305442
Abstract: Human lung tissue-resident NK cells (trNK cells) are likely to play an important role in host responses towards viral infections, inflammatory conditions and cancer. However, detailed insights into these cells are still largely lacking. Here we show, using RNA sequencing and flow cytometry-based analyses, that subsets of human lung CD69(-)CD16(-) NK cells display hallmarks of tissue-residency, including high expression of CD49a, CD103, and ZNF683, and reduced expression of SELL, S1PR5, and KLF2/3. CD49a(+)CD16(-) NK cells are functionally competent, and produce IFN-gamma, TNF, MIP-1 beta, and GM-CSF. After stimulation with IL-15, they upregulate perforin, granzyme B, and Ki67 to a similar degree as CD49a(-) CD16(-) NK cells. Comparing datasets from trNK cells in human lung and bone marrow with tissue-resident memory CD8(+) T cells identifies core genes co-regulated either by tissue-residency, cell-type or location. Together, our data indicate that human lung trNK cells have distinct features, likely regulating their function in barrier immunity.
Subject: NATURAL-KILLER-CELLS
MEMORY T-CELLS
CYTOTOXIC FUNCTION
IFN-GAMMA
IL-15
INFECTION
SUBSET
INDUCE
CXCR6
MIGRATION
3111 Biomedicine
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