Selective autophagy of mitochondria on a ubiquitin-endoplasmic reticulum platform

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dc.contributor.author Zachari, Maria
dc.contributor.author Gudmundsson, Sigurdur R.
dc.contributor.author Li, Ziyue
dc.contributor.author Manifava, Maria
dc.contributor.author Shah, Ronak
dc.contributor.author Smith, Matthew
dc.contributor.author Stronge, James
dc.contributor.author Karanasios, Eleftherios
dc.contributor.author Piunti, Caterina
dc.contributor.author Kishi-Itakura, Chieko
dc.contributor.author Vihinen, Helena
dc.contributor.author Jokitalo, Eija
dc.contributor.author Guan, Jun-Lin
dc.contributor.author Buss, Folma
dc.contributor.author Smith, Andrew M.
dc.contributor.author Walker, Simon A.
dc.contributor.author Eskelinen, Eeva-Liisa
dc.contributor.author Ktistakis, Nicholas T.
dc.date.accessioned 2019-10-02T13:37:02Z
dc.date.available 2019-10-02T13:37:02Z
dc.date.issued 2019-09-09
dc.identifier.citation Zachari , M , Gudmundsson , S R , Li , Z , Manifava , M , Shah , R , Smith , M , Stronge , J , Karanasios , E , Piunti , C , Kishi-Itakura , C , Vihinen , H , Jokitalo , E , Guan , J-L , Buss , F , Smith , A M , Walker , S A , Eskelinen , E-L & Ktistakis , N T 2019 , ' Selective autophagy of mitochondria on a ubiquitin-endoplasmic reticulum platform ' , Developmental Cell , vol. 50 , no. 5 , pp. 627-+ . https://doi.org/10.1016/j.devcel.2019.06.016
dc.identifier.other PURE: 125304510
dc.identifier.other PURE UUID: ed8aa7ee-e37d-47da-832a-2f9c354b6d1d
dc.identifier.other WOS: 000484741700012
dc.identifier.other ORCID: /0000-0003-3862-9237/work/62631593
dc.identifier.other ORCID: /0000-0003-0006-7785/work/62631671
dc.identifier.other ORCID: /0000-0002-4159-6934/work/62633203
dc.identifier.other WOS: 000582501100016
dc.identifier.uri http://hdl.handle.net/10138/305804
dc.description Correction: Developmental Cell, Volume 55, Issue 2 https://doi.org/10.1016/j.devcel.2020.10.002
dc.description.abstract The dynamics and co-ordination between autophagy machinery and selective receptors during mitophagy are unknown. Also unknown is whether mitophagy depends on pre-existing membranes, or is triggered on the surface of damaged mitochondria. Using a ubiquitin-dependent mitophagy inducer, the lactone ivermectin, we have combined genetic and imaging experiments to address these questions. Ubiquitination of mitochondrial fragments is required earliest followed by autophosphorylation of TBK1. Next, early essential autophagy proteins FIP200 and ATG13 act at different steps whereas ULK1/2 are dispensable. Receptors act temporally and mechanistically upstream of ATG13 but downstream of FIP200. The VPS34 complex functions at the omegasome step. ATG13 and optineurin target mitochondria in a discontinuous oscillatory way suggesting multiple initiation events. Targeted ubiquitinated mitochondrial are cradled by endoplasmic reticulum strands even without functional autophagy machinery and mitophagy adaptors. We propose that damaged mitochondria are ubiquitinated and dynamically encased in ER strands providing platforms for formation of the mitophagosomes. en
dc.format.extent 22
dc.language.iso eng
dc.relation.ispartof Developmental Cell
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject 1182 Biochemistry, cell and molecular biology
dc.subject autophagy
dc.subject mitophagy
dc.subject ivermectin
dc.subject ubiquitin
dc.subject endoplasmic reticulum
dc.title Selective autophagy of mitochondria on a ubiquitin-endoplasmic reticulum platform en
dc.type Article
dc.contributor.organization Autophagy
dc.contributor.organization External Funding
dc.contributor.organization Biosciences
dc.contributor.organization Institute of Biotechnology
dc.contributor.organization Electron Microscopy
dc.contributor.organization University Management
dc.contributor.organization Molecular and Integrative Biosciences Research Programme
dc.contributor.organization Biochemistry and Biotechnology
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1016/j.devcel.2019.06.016
dc.relation.issn 1534-5807
dc.rights.accesslevel openAccess
dc.type.version publishedVersion
dc.relation.funder European Union
dc.relation.funder Acedemy of Finland
dc.relation.grantnumber
dc.relation.grantnumber

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