Dynamics of Protein Accumulation from the 3 ' End of Viral RNA Are Different from Those in the Rest of the Genome in Potato Virus A Infection

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Saha , S , Hafren , A & Makinen , K 2019 , ' Dynamics of Protein Accumulation from the 3 ' End of Viral RNA Are Different from Those in the Rest of the Genome in Potato Virus A Infection ' , Journal of Virology , vol. 93 , no. 19 , e00721-19 . https://doi.org/10.1128/JVI.00721-19

Title: Dynamics of Protein Accumulation from the 3 ' End of Viral RNA Are Different from Those in the Rest of the Genome in Potato Virus A Infection
Author: Saha, Shreya; Hafren, Anders; Makinen, Kristiina
Contributor: University of Helsinki, Department of Microbiology
University of Helsinki, Plant-Virus Interactions
University of Helsinki, Department of Microbiology
Date: 2019-10
Language: eng
Number of pages: 18
Belongs to series: Journal of Virology
ISSN: 0022-538X
URI: http://hdl.handle.net/10138/305859
Abstract: One large open reading frame (ORF) encodes 10 potyviral proteins. We compared the accumulation of cylindrical inclusion (CI) protein from the middle, coat protein (CP) from the 3'end, and Renilla luciferase (RLUC) from two distinct locations in potato virus A (PVA) RNA. 5' RLUC was expressed from an rluc gene inserted between the P1 and helper component proteinase (HCPro) cistrons, and 3' RLUC was expressed from the gene inserted between the RNA polymerase and CP cistrons. Viral protein and RNA accumulation were quantitated (i) when expressed from PVA RNA in the presence of ectopically expressed genome-linked viral protein (VPg) and auxiliary proteins and (ii) at different time points during natural infection. The rate and timing of 3' RLUC and CP accumulation were found to be different from those of 5' RLUC and Cl. Ectopic expression of VPg boosted PVA RNA, 3' RLUC, and, together with HCPro, CP accumulation, whereas 5' RLUC and CI accumulation remained unaffected regardless of the increased viral RNA amount. In natural infection, the rate of the noteworthy minute early accumulation of 3' RLUC accelerated toward the end of infection. 5' RLUC accumulation, which was already pronounced at 2 days postinfection, increased moderately and stabilized to a constant level by day 5, whereas PVA RNA and CP levels continued to increase throughout the infection. We propose that these observations connect with the mechanisms by which potyvirus infection limits CP accumulation during early infection and specifically supports its accumulation late in infection, but follow-up studies are required to understand the mechanism of how this occurs. IMPORTANCE The results of this study suggest that the dynamics of potyviral protein accumulation are regulated differentially from the 3' end of viral RNA than from the rest of the genome, the significance of which would be to satisfy the needs of replication early and particle assembly late in infection.
Subject: helper component proteinase
HCPro
polyprotein
potyvirus
ribosomal protein P0
stoichiometry of protein production
translation
genome-linked viral protein
VPg
TRANSLATION INITIATION-FACTORS
RECESSIVE RESISTANCE
PLANT-VIRUSES
POTYVIRUS
HIV-1
VPG
BENTHAMIANA
LUCIFERASE
SLIPPAGE
INSIGHTS
1183 Plant biology, microbiology, virology
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